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Journal of Bacteriology, August 2003, p. 5023-5026, Vol. 185, No. 16
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.16.5023-5026.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Conversion of Lactobacillus pentosus D-Lactate Dehydrogenase to a D-Hydroxyisocaproate Dehydrogenase through a Single Amino Acid Replacement

Department of Applied Biological Science, Faculty of Science and Technology, Science University of Tokyo, Noda, Chiba 278-8510, Japan

Received 21 March 2003/ Accepted 28 May 2003

The single amino acid replacement of Tyr52 with Leu drastically increased the activity of Lactobacillus pentosus NAD-dependent D-lactate dehydrogenase toward larger aliphatic or aromatic 2-ketoacid substrates by 3 or 4 orders of magnitude and decreased the activity toward pyruvate by about 30-fold, converting the enzyme into a highly active D-2-hydroxyisocaproate dehydrogenase.

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