This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Taniya, T.
Right arrow Articles by Watanabe, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Taniya, T.
Right arrow Articles by Watanabe, H.

 Previous Article  |  Next Article 

Journal of Bacteriology, September 2003, p. 5158-5165, Vol. 185, No. 17
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.17.5158-5165.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Determination of the InvE Binding Site Required for Expression of IpaB of the Shigella sonnei Virulence Plasmid: Involvement of a ParB BoxA-Like Sequence

Takayuki Taniya,1,2 Jiro Mitobe,1 Shu-ichi Nakayama,1 Qi Mingshan,3 Kenji Okuda,2 and Haruo Watanabe1*

Department of Bacteriology, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku-ku, Tokyo 162-8640,1 Department of Bacteriology, Yokohama City University School of Medicine, Fukuura 3 to 9, Kanazawa-ku, Yokohama 236-0004, Japan,2 Department of Oral Biology, School of Dental Medicine, Buffalo, New York 14214-30923

Received 20 March 2003/ Accepted 16 June 2003

The InvE protein positively regulates the expression of virulence genes ipaBCD in Shigella sonnei. The InvE has significant homology with ParB of plasmid P1, which is known as a plasmid partitioning factor with DNA binding ability. Although the DNA binding activity of InvE has been predicted, it is not known whether the DNA binding activity is necessary for type III secretion system-associated gene expression. In this study, we determined the transcription start site of the icsB-ipaBCD operon (ipa operon) and constructed a series of deletions of the icsB promoter region in the Escherichia coli K-12 background. The deletion study revealed that an 86-bp region upstream of the icsB transcription start site was essential for expression of the ipa operon, where the ParB binding motif (ParB BoxA-like sequence) was observed. Purified glutathione S-transferase-InvE fusion protein bound directly to the -93 to -54 region (designating the icsB transcription start site as nucleotide +1) containing the ParB BoxA-like sequence. These results indicated that InvE bound directly to the promoter region.

* Corresponding author. Mailing address: Department of Bacteriology, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku-ku, Tokyo 162-8640, Japan. Phone: (81 3) 5285 1111, ext. 2201. Fax: (81 3) 5285 1171. E-mail: haruwata{at}nih.go.jp.

Journal of Bacteriology, September 2003, p. 5158-5165, Vol. 185, No. 17
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.17.5158-5165.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Castellanos, M. I., Harrison, D. J., Smith, J. M., Labahn, S. K., Levy, K. M., Wing, H. J. (2009). VirB Alleviates H-NS Repression of the icsP Promoter in Shigella flexneri from Sites More Than One Kilobase Upstream of the Transcription Start Site. J. Bacteriol. 191: 4047-4050 [Abstract] [Full Text]  
  • Stoebel, D. M., Free, A., Dorman, C. J. (2008). Anti-silencing: overcoming H-NS-mediated repression of transcription in Gram-negative enteric bacteria. Microbiology 154: 2533-2545 [Abstract] [Full Text]  
  • Mitobe, J., Morita-Ishihara, T., Ishihama, A., Watanabe, H. (2008). Involvement of RNA-binding Protein Hfq in the Post-transcriptional Regulation of invE Gene Expression in Shigella sonnei. J. Biol. Chem. 283: 5738-5747 [Abstract] [Full Text]  
  • Turner, E. C., Dorman, C. J. (2007). H-NS Antagonism in Shigella flexneri by VirB, a Virulence Gene Transcription Regulator That Is Closely Related to Plasmid Partition Factors. J. Bacteriol. 189: 3403-3413 [Abstract] [Full Text]  
  • Gall, T. L., Mavris, M., Martino, M. C., Bernardini, M. L., Denamur, E., Parsot, C. (2005). Analysis of virulence plasmid gene expression defines three classes of effectors in the type III secretion system of Shigella flexneri. Microbiology 151: 951-962 [Abstract] [Full Text]  
  • Mitobe, J., Arakawa, E., Watanabe, H. (2005). A Sensor of the Two-Component System CpxA Affects Expression of the Type III Secretion System through Posttranscriptional Processing of InvE. J. Bacteriol. 187: 107-113 [Abstract] [Full Text]  
  • Wing, H. J., Yan, A. W., Goldman, S. R., Goldberg, M. B. (2004). Regulation of IcsP, the Outer Membrane Protease of the Shigella Actin Tail Assembly Protein IcsA, by Virulence Plasmid Regulators VirF and VirB. J. Bacteriol. 186: 699-705 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»