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Journal of Bacteriology, September 2003, p. 5166-5174, Vol. 185, No. 17
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.17.5166-5174.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Role of RopB in Growth Phase Expression of the SpeB Cysteine Protease of Streptococcus pyogenes

Melody N. Neely,1* William R. Lyon,2 Donna L. Runft,1 and Michael Caparon2

Department of Immunology and Microbiology, Wayne State University School of Medicine, Detroit, Michigan 48201,1 Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri 63110-10932

Received 7 April 2003/ Accepted 18 June 2003

The Rgg family of transcription regulators is widely distributed among gram-positive bacteria; however, how the members of this family control transcription is poorly understood. In the pathogen Streptococcus pyogenes, the Rgg family member RopB is required for transcription of the gene that encodes the secreted SpeB cysteine protease. Expression of the protease follows distinct kinetics that involves control of transcription in response to the growth phase. In this study, the contribution of RopB to growth phase control was examined. The gene encoding the protease (speB) and ropB are transcribed divergently from a 940-bp intergenic region. Primer extension analyses, in conjunction with reporter fusion studies, revealed that the major region controlling the transcription of both speB and ropB is adjacent to ropB and that the promoters for the two genes likely overlap. Furthermore, it was found that RopB is a DNA-binding protein that specifically binds to sequences in this control region. The interrelationship between ropB and speB expression was further reflected in the observation that transcription of ropB itself is subject to growth phase control. However, while expression of ropB from a promoter expressed during the early logarithmic phase of growth could complement a ropB deletion mutant, ectopic expression of ropB did not uncouple the expression of speB from its growth phase signal. These data implicate other factors in growth phase control and suggest that regulation of ropB expression itself is not the central mechanism of control.

* Corresponding author. Mailing address: Department of Immunology and Microbiology, Wayne State University School of Medicine, 540 East Canfield Ave., Detroit, MI 48201. Phone: (313) 577-1314. Fax: (313) 577-1155. E-mail: mneely{at}med.wayne.edu.

Journal of Bacteriology, September 2003, p. 5166-5174, Vol. 185, No. 17
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.17.5166-5174.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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