This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Neely, M. N.
Right arrow Articles by Caparon, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Neely, M. N.
Right arrow Articles by Caparon, M.

 Previous Article  |  Next Article 

Journal of Bacteriology, September 2003, p. 5166-5174, Vol. 185, No. 17
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.17.5166-5174.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Role of RopB in Growth Phase Expression of the SpeB Cysteine Protease of Streptococcus pyogenes

Melody N. Neely,1* William R. Lyon,2 Donna L. Runft,1 and Michael Caparon2

Department of Immunology and Microbiology, Wayne State University School of Medicine, Detroit, Michigan 48201,1 Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri 63110-10932

Received 7 April 2003/ Accepted 18 June 2003

The Rgg family of transcription regulators is widely distributed among gram-positive bacteria; however, how the members of this family control transcription is poorly understood. In the pathogen Streptococcus pyogenes, the Rgg family member RopB is required for transcription of the gene that encodes the secreted SpeB cysteine protease. Expression of the protease follows distinct kinetics that involves control of transcription in response to the growth phase. In this study, the contribution of RopB to growth phase control was examined. The gene encoding the protease (speB) and ropB are transcribed divergently from a 940-bp intergenic region. Primer extension analyses, in conjunction with reporter fusion studies, revealed that the major region controlling the transcription of both speB and ropB is adjacent to ropB and that the promoters for the two genes likely overlap. Furthermore, it was found that RopB is a DNA-binding protein that specifically binds to sequences in this control region. The interrelationship between ropB and speB expression was further reflected in the observation that transcription of ropB itself is subject to growth phase control. However, while expression of ropB from a promoter expressed during the early logarithmic phase of growth could complement a ropB deletion mutant, ectopic expression of ropB did not uncouple the expression of speB from its growth phase signal. These data implicate other factors in growth phase control and suggest that regulation of ropB expression itself is not the central mechanism of control.

* Corresponding author. Mailing address: Department of Immunology and Microbiology, Wayne State University School of Medicine, 540 East Canfield Ave., Detroit, MI 48201. Phone: (313) 577-1314. Fax: (313) 577-1155. E-mail: mneely{at}med.wayne.edu.

Journal of Bacteriology, September 2003, p. 5166-5174, Vol. 185, No. 17
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.17.5166-5174.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Kappeler, K. V., Anbalagan, S., Dmitriev, A. V., McDowell, E. J., Neely, M. N., Chaussee, M. S. (2009). A Naturally Occurring Rgg Variant in Serotype M3 Streptococcus pyogenes Does Not Activate speB Expression Due to Altered Specificity of DNA Binding. Infect. Immun. 77: 5411-5417 [Abstract] [Full Text]  
  • Kizy, A. E., Neely, M. N. (2009). First Streptococcus pyogenes Signature-Tagged Mutagenesis Screen Identifies Novel Virulence Determinants. Infect. Immun. 77: 1854-1865 [Abstract] [Full Text]  
  • Ma, Y., Bryant, A. E., Salmi, D. B., McIndoo, E., Stevens, D. L. (2009). vfr, a Novel Locus Affecting Cysteine Protease Production in Streptococcus pyogenes. J. Bacteriol. 191: 3189-3194 [Abstract] [Full Text]  
  • Phelps, H. A., Neely, M. N. (2007). SalY of the Streptococcus pyogenes Lantibiotic Locus Is Required for Full Virulence and Intracellular Survival in Macrophages. Infect. Immun. 75: 4541-4551 [Abstract] [Full Text]  
  • Sawai, J., Hasegawa, T., Kamimura, T., Okamoto, A., Ohmori, D., Nosaka, N., Yamada, K., Torii, K., Ohta, M. (2007). Growth Phase-Dependent Effect of Clindamycin on Production of Exoproteins by Streptococcus pyogenes. Antimicrob. Agents Chemother. 51: 461-467 [Abstract] [Full Text]  
  • Loughman, J. A., Caparon, M. G. (2007). Contribution of Invariant Residues to the Function of Rgg Family Transcription Regulators. J. Bacteriol. 189: 650-655 [Abstract] [Full Text]  
  • Ma, Y., Bryant, A. E., Salmi, D. B., Hayes-Schroer, S. M., McIndoo, E., Aldape, M. J., Stevens, D. L. (2006). Identification and Characterization of Bicistronic speB and prsA Gene Expression in the Group A Streptococcus. J. Bacteriol. 188: 7626-7634 [Abstract] [Full Text]  
  • Bigot, A., Botton, E., Dubail, I., Charbit, A. (2006). A Homolog of Bacillus subtilis Trigger Factor in Listeria monocytogenes Is Involved in Stress Tolerance and Bacterial Virulence.. Appl. Environ. Microbiol. 72: 6623-6631 [Abstract] [Full Text]  
  • Samen, U. M., Eikmanns, B. J., Reinscheid, D. J. (2006). The Transcriptional Regulator RovS Controls the Attachment of Streptococcus agalactiae to Human Epithelial Cells and the Expression of Virulence Genes.. Infect. Immun. 74: 5625-5635 [Abstract] [Full Text]  
  • Dmitriev, A. V., McDowell, E. J., Kappeler, K. V., Chaussee, M. A., Rieck, L. D., Chaussee, M. S. (2006). The Rgg Regulator of Streptococcus pyogenes Influences Utilization of Nonglucose Carbohydrates, Prophage Induction, and Expression of the NAD-Glycohydrolase Virulence Operon.. J. Bacteriol. 188: 7230-7241 [Abstract] [Full Text]  
  • Chaussee, M. A., McDowell, E. J., Rieck, L. D., Callegari, E. A., Chaussee, M. S. (2006). Proteomic analysis of a penicillin-tolerant rgg mutant strain of Streptococcus pyogenes. J Antimicrob Chemother 58: 752-759 [Abstract] [Full Text]  
  • Loughman, J. A., Caparon, M. (2006). Regulation of SpeB in Streptococcus pyogenes by pH and NaCl: a Model for In Vivo Gene Expression. J. Bacteriol. 188: 399-408 [Abstract] [Full Text]  
  • Rawlinson, E. L. A., Nes, I. F., Skaugen, M. (2005). Identification of the DNA-binding site of the Rgg-like regulator LasX within the lactocin S promoter region. Microbiology 151: 813-823 [Abstract] [Full Text]  
  • Zimmerlein, B., Park, H.-S., Li, S., Podbielski, A., Cleary, P. P. (2005). The M Protein Is Dispensable for Maturation of Streptococcal Cysteine Protease SpeB. Infect. Immun. 73: 859-864 [Abstract] [Full Text]  
  • Chaussee, M. A., Callegari, E. A., Chaussee, M. S. (2004). Rgg Regulates Growth Phase-Dependent Expression of Proteins Associated with Secondary Metabolism and Stress in Streptococcus pyogenes. J. Bacteriol. 186: 7091-7099 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»