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Journal of Bacteriology, September 2003, p. 5234-5239, Vol. 185, No. 17
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.17.5234-5239.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Topology of RbsC, the Membrane Component of the Escherichia coli Ribose Transporter{dagger}

Jeffrey B. Stewart and Mark A. Hermodson*

Department of Biochemistry, Purdue University, West Lafayette, Indiana

Received 9 April 2003/ Accepted 12 June 2003

The topology of RbsC, the membrane component of the ribose transporter in Escherichia coli, has been determined by using 34 single-cysteine mutants and a modified fluorescence labeling technique designated multiplex labeling. This technique gives topology, expression, and localization information for a membrane protein from a single batch of bacterial cells. The results indicate that RbsC contains 10 transmembrane-spanning helices, with the N and C termini being in the cytosol. This topology matches predictions from the latest prediction programs and the topology of the similar, recently crystallized membrane protein BtuC.

* Corresponding author. Mailing address: Department of Biochemistry, Purdue University, 175 S. University St., West Lafayette, IN 47907. Phone: (765) 494-1637. Fax: (765) 494-7897. E-mail: hermodson{at}purdue.edu.

{dagger} Journal paper no. 17113 of the Purdue Agricultural Experiment Station.

Journal of Bacteriology, September 2003, p. 5234-5239, Vol. 185, No. 17
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.17.5234-5239.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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