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Journal of Bacteriology, September 2003, p. 5279-5286, Vol. 185, No. 17
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.17.5279-5286.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Polynucleotide Phosphorylase-Deficient Mutants of Pseudomonas putida

Rebecca Favaro{dagger} and Gianni Dehò*

Dipartimento di Genetica e di Biologia dei Microrganismi, Università degli Studi di Milano, 20133 Milan, Italy

Received 24 February 2003/ Accepted 6 June 2003

In bacteria, polynucleotide phosphorylase (PNPase) is one of the main exonucleolytic activities involved in RNA turnover and is widely conserved. In spite of this, PNPase does not seem to be essential for growth if the organisms are not subjected to special conditions, such as low temperature. We identified the PNPase-encoding gene (pnp) of Pseudomonas putida and constructed deletion mutants that did not exhibit cold sensitivity. In addition, we found that the transcription pattern of pnp upon cold shock in P. putida was markedly different from that in Escherichia coli. It thus appears that pnp expression control and the physiological roles in the cold may be different in different bacterial species.

* Corresponding author. Mailing address: Dipartimento di Scienze biomolecolari e Biotecnologie, Università degli Studi di Milano, Via Celoria 26, 20133 Milan, Italy. Phone: (39)02 5031 5019. Fax: (39)02 5031 5044. E-mail: gianni.deho{at}unimi.it.

{dagger} Present address: Dipartimento di Biotecnologie e Bioscienze, Università di Milano Bicocca, Milan, Italy.

Journal of Bacteriology, September 2003, p. 5279-5286, Vol. 185, No. 17
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.17.5279-5286.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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