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FNR-Mediated Oxygen-Responsive Regulation of the nrdDG Operon of Escherichia coli

Department of Life Sciences and Chemistry, Roskilde University, DK-4000 Roskilde,¹ Novo Nordisk IT A/S, DK-2880 Bagsværd, Denmark²

Received 13 January 2003/ Accepted 12 June 2003

Transcription of the nrdDG operon, which encodes the class III nucleotide reductase, which is only active under anaerobic conditions, was strongly induced after a shift to anaerobiosis. The induction was completely dependent on the transcriptional activator FNR and was independent of the ArcA-ArcB two-component response regulator system. The nrdD transcript start site was mapped to a position immediately downstream of two FNR binding sites. Transcription of the other two nucleotide reductase operons, nrdAB and nrdEF, did not respond to oxygen conditions in a wild-type background, but nrdAB expression was increased in the fnr mutant under anaerobic conditions.

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