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Journal of Bacteriology, September 2003, p. 5320-5323, Vol. 185, No. 17
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.17.5320-5323.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Integration Site for Streptomyces Phage {phi}BT1 and Development of Site-Specific Integrating Vectors

Matthew A. Gregory,{dagger} Rob Till, and Margaret C. M. Smith*

Institute of Genetics, University of Nottingham, Queens Medical Centre, Nottingham NG7 2UH, United Kingdom

Received 3 March 2003/ Accepted 15 June 2003

Despite extensive similarities between the genomes of the Streptomyces temperate phages {phi}C31 and {phi}BT1, the attP-int loci are poorly conserved. Here we demonstrate that {phi}BT1 integrates into a different attachment site than {phi}C31. {phi}BT1 attB lies within SCO4848 encoding a 79-amino-acid putative integral membrane protein. Integration vectors based on {phi}BT1 integrase were shown to have a broad host range and are fully compatible with those based on the {phi}C31 attP-int locus.

* Corresponding author. Mailing address: Institute of Genetics, University of Nottingham, Queens Medical Centre, Nottingham NG7 2UH, United Kingdom. Phone: (44) 115 970 9778. Fax: (44) 115 970 9906. E-mail: maggie.smith{at}nottingham.ac.uk.

{dagger} Present address: Biotica, 181A Huntingdon Rd., Cambridge, CB3 0DJ, United Kingdom.

Journal of Bacteriology, September 2003, p. 5320-5323, Vol. 185, No. 17
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.17.5320-5323.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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