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Integration Site for Streptomyces Phage BT1 and Development of Site-Specific Integrating Vectors

Matthew A. Gregory, Rob Till, and Margaret C. M. Smith^*

Institute of Genetics, University of Nottingham, Queens Medical Centre, Nottingham NG7 2UH, United Kingdom

Received 3 March 2003/ Accepted 15 June 2003

Despite extensive similarities between the genomes of the Streptomyces temperate phages C31 and BT1, the attP-int loci are poorly conserved. Here we demonstrate that BT1 integrates into a different attachment site than C31. BT1 attB lies within SCO4848 encoding a 79-amino-acid putative integral membrane protein. Integration vectors based on BT1 integrase were shown to have a broad host range and are fully compatible with those based on the C31 attP-int locus.

Present address: Biotica, 181A Huntingdon Rd., Cambridge, CB3 0DJ, United Kingdom.

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