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 Previous Article

Journal of Bacteriology, September 2003, p. 5328-5332, Vol. 185, No. 17
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.17.5328-5332.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Role for Salmonella enterica Enterobacterial Common Antigen in Bile Resistance and Virulence

Francisco Ramos-Morales,1* Ana I. Prieto,1 Carmen R. Beuzón,2,{dagger} David W. Holden,2 and Josep Casadesús1

Departamento de Genética, Facultad de Biología, Universidad de Sevilla, Seville 41080, Spain,1 Department of Infectious Diseases, Centre for Molecular Microbiology and Infection, Imperial College School of Medicine, London SW7 2AZ, United Kingdom2

Received 17 March 2003/ Accepted 2 June 2003

Passage through the digestive tract exposes Salmonella enterica to high concentrations of bile salts, powerful detergents that disrupt biological membranes. Mutations in the wecD or wecA gene, both of which are involved in the synthesis of enterobacterial common antigen (ECA), render S. enterica serovar Typhimurium sensitive to the bile salt deoxycholate. Competitive infectivity analysis of wecD and wecA mutants in the mouse model indicates that ECA is an important virulence factor for oral infection. In contrast, lack of ECA causes only a slight decrease in Salmonella virulence during intraperitoneal infection. A tentative interpretation is that ECA may contribute to Salmonella virulence by protecting the pathogen from bile salts.

* Corresponding author. Mailing address: Departamento de Genética, Facultad de Biología, Universidad de Sevilla, Apdo. 1095, 41080 Seville, Spain. Phone: 34 954557106. Fax: 34 954557104. E-mail: framos{at}us.es.

{dagger} Present address: Dep. Biología Celular, Genética y Fisiología, Universidad de Málaga, Campus de Teatinos, 29071 Málaga, Spain.

Journal of Bacteriology, September 2003, p. 5328-5332, Vol. 185, No. 17
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.17.5328-5332.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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