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Journal of Bacteriology, October 2003, p. 5897-5900, Vol. 185, No. 19
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.19.5897-5900.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Tethering of the Bacillus subtilis {sigma}E Proprotein to the Cell Membrane Is Necessary for Its Processing but Insufficient for Its Stabilization

Jingliang Ju{dagger} and W. G. Haldenwang*

Department of Microbiology & Immunology, University of Texas Health Science Center, San Antonio, Texas 78229-3900

Received 8 May 2003/ Accepted 7 July 2003

{sigma}E, a sporulation-specific transcription factor of Bacillus subtilis, is synthesized as an inactive proprotein with a 27-amino acid extension at its amino terminus. This "pro" sequence is removed by a developmentally regulated protease, but when present, it blocks {sigma}E activity, tethers {sigma}E to the bacterium's cytoplasmic membrane, and promotes {sigma}E stability. To investigate whether pro-{sigma}E processing and/or stabilization are tied to membrane sequestration, we used fluorescent protein fusions to examine the membrane binding of SigE variants. The results are consistent with membrane association as a prerequisite for pro-{sigma}E processing but not as a sufficient cause for the proprotein's stability.

* Corresponding author. Mailing address: Dept. of Microbiology & Immunology MC7758, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Dr., San Antonio, TX 78229-3900. Phone: (210) 567-3957. Fax: (210) 567-6612. E-mail: Haldenwang{at}UTHSCSA.EDU.

{dagger} Present address: Adaptive Therapeutics, Inc., San Diego, CA 92121.

Journal of Bacteriology, October 2003, p. 5897-5900, Vol. 185, No. 19
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.19.5897-5900.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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  • Hilbert, D. W., Piggot, P. J. (2004). Compartmentalization of Gene Expression during Bacillus subtilis Spore Formation. Microbiol. Mol. Biol. Rev. 68: 234-262 [Abstract] [Full Text]  
  • McBride, S., Haldenwang, W. G. (2004). Sporulation Phenotype of a Bacillus subtilis Mutant Expressing an Unprocessable but Active {sigma}E Transcription Factor. J. Bacteriol. 186: 1999-2005 [Abstract] [Full Text]  


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