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Journal of Bacteriology, October 2003, p. 5901-5905, Vol. 185, No. 19
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.19.5901-5905.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Global Regulation of the Salmonella enterica Serovar Typhimurium Major Porin, OmpD

Carlos A. Santiviago,¹ Cecilia S. Toro,² Alejandro A. Hidalgo,¹ Philip Youderian,³ and Guido C. Mora^1*

Unidad de Microbiología, Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile,¹ Programa de Microbiología y Micología, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago, Chile,² Department of Biology, Texas A&M University, College Station, Texas 83843-3052³

Received 31 March 2003/ Accepted 9 July 2003

The OmpD porin is the most abundant outer membrane protein in Salmonella enterica serovar Typhimurium and represents about 1% of total cell protein. Unlike the case with the less abundant OmpC and OmpF porins, the stoichiometry of OmpD in the outer membrane does not change in response to changes in osmolarity. The abundance of OmpD increases in response to anaerobiosis and decreases in response to low pH, conditions encountered by serovar Typhimurium during the infection of its murine host. By constructing an operon fusion of the lacZY genes with the ompD promoter, we show that the abundance of OmpD in the outer membrane is regulated primarily at the level of transcription and is subject to catabolite repression. In response to anaerobiosis, the abundance of OmpD in the outer membrane also appears to be controlled posttranscriptionally by a function dependent on Fnr.

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* Corresponding author. Mailing address: Unidad de Microbiología, Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Alameda 340, Santiago, Chile. Phone: (56) 2686 2847. Fax: (56) 2222 5515. E-mail: gmora{at}genes.bio.puc.cl.

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Journal of Bacteriology, October 2003, p. 5901-5905, Vol. 185, No. 19
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.19.5901-5905.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

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