This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Xiang, L.
Right arrow Articles by Moore, B. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Xiang, L.
Right arrow Articles by Moore, B. S.

 Previous Article  |  Next Article 

Journal of Bacteriology, January 2003, p. 399-404, Vol. 185, No. 2
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.2.399-404.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Characterization of Benzoyl Coenzyme A Biosynthesis Genes in the Enterocin-Producing Bacterium "Streptomyces maritimus"

Longkuan Xiang1 and Bradley S. Moore1,2*

College of Pharmacy the,1 Department of Chemistry, University of Arizona, Tucson, Arizona 857212

Received 26 June 2002/ Accepted 6 October 2002

The novel benzoyl coenzyme A (benzoyl-CoA) biosynthesis pathway in "Streptomyces maritimus" was investigated through a series of target-directed mutations. Genes involved in benzoyl-CoA formation were disrupted through single-crossover homologous recombination, and the resulting mutants were analyzed for their ability to biosynthesize the benzoyl-CoA-primed polyketide antibiotic enterocin. Inactivation of the unique phenylalanine ammonia-lyase-encoding gene encP was previously shown to be absolutely required for benzoyl-CoA formation in "S. maritimus". The fatty acid ß-oxidation-related genes encH, -I, and -J, on the other hand, are necessary but not required. In each case, the yield of benzoyl-CoA-primed enterocin dropped below wild-type levels. We attribute the reduced benzoyl-CoA formation in these specific mutants to functional substitution and cross-talk between the products of genes encH, -I, and -J and the enzyme homologues of primary metabolism. Disruption of the benzoate-CoA ligase encN gene did not perturb enterocin production, however, demonstrating that encN is extraneous and that benzoic acid is not a pathway intermediate. EncN rather serves as a substitute pathway for utilizing exogenous benzoic acid. These experiments provide further support that benzoyl-CoA is formed in a novel bacterial pathway that resembles the eukaryotic assembly of benzoyl-CoA from phenylalanine via a ß-oxidative path.

* Corresponding author. Mailing address: College of Pharmacy, 1703 E. Mabel St., University of Arizona, Tucson, AZ 85721-0207. Phone: (520) 626-6931. Fax: (520) 626-2466. E-mail: moore{at}pharmacy.arizona.edu.

Journal of Bacteriology, January 2003, p. 399-404, Vol. 185, No. 2
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.2.399-404.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Carmona, M., Zamarro, M. T., Blazquez, B., Durante-Rodriguez, G., Juarez, J. F., Valderrama, J. A., Barragan, M. J. L., Garcia, J. L., Diaz, E. (2009). Anaerobic Catabolism of Aromatic Compounds: a Genetic and Genomic View. Microbiol. Mol. Biol. Rev. 73: 71-133 [Abstract] [Full Text]  
  • Peng, X., Adachi, K., Chen, C., Kasai, H., Kanoh, K., Shizuri, Y., Misawa, N. (2006). Discovery of a Marine Bacterium Producing 4-Hydroxybenzoate and Its Alkyl Esters, Parabens. Appl. Environ. Microbiol. 72: 5556-5561 [Abstract] [Full Text]  
  • Williams, J. S., Thomas, M., Clarke, D. J. (2005). The gene stlA encodes a phenylalanine ammonia-lyase that is involved in the production of a stilbene antibiotic in Photorhabdus luminescens TT01. Microbiology 151: 2543-2550 [Abstract] [Full Text]  
  • Xiang, L., Moore, B. S. (2005). Biochemical Characterization of a Prokaryotic Phenylalanine Ammonia Lyase. J. Bacteriol. 187: 4286-4289 [Abstract] [Full Text]  
  • Xiang, L., Kalaitzis, J. A., Moore, B. S. (2004). EncM, a versatile enterocin biosynthetic enzyme involved in Favorskii oxidative rearrangement, aldol condensation, and heterocycle-forming reactions. Proc. Natl. Acad. Sci. USA 101: 15609-15614 [Abstract] [Full Text]  
  • Zirkle, R., Ligon, J. M., Molnar, I. (2004). Heterologous production of the antifungal polyketide antibiotic soraphen A of Sorangium cellulosum So ce26 in Streptomyces lividans. Microbiology 150: 2761-2774 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»