JB
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Saïd-Salim, B.
Right arrow Articles by Kreiswirth, B. N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Saïd-Salim, B.
Right arrow Articles by Kreiswirth, B. N.
Journal of Bacteriology, January 2003, p. 610-619, Vol. 185, No. 2
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.2.610-619.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Global Regulation of Staphylococcus aureus Genes by Rot

B. Saïd-Salim,1,2 P. M. Dunman,3 F. M. McAleese,4 D. Macapagal,3 E. Murphy,5 P. J. McNamara,6 S. Arvidson,7 T. J. Foster,4 S. J. Projan,3 and B. N. Kreiswirth1*

Public Health Research Institute at the International Center of Public Health, Newark, New Jersey, 07103,1 Department of Microbiology, New York University School of Medicine, New York, New York 10016,2 Department of Infectious Diseases,3 Bioinformatics, Wyeth Research, Pearl River, New York 10965,5 Microbiology Department, Moyne Institute for Preventive Medicine, Trinity College, Dublin 2, Ireland,4 Department of Medical Microbiology and Immunology, University of Wisconsin Medical School, Madison, Wisconsin 53706,6 Microbiology and Tumor Biology Center, Karolinska Institute, S-17177 Stockholm, Sweden7

Received 2 August 2002/ Accepted 17 October 2002

Staphylococcus aureus produces a wide array of cell surface and extracellular proteins involved in virulence. Expression of these virulence factors is tightly controlled by numerous regulatory loci, including agr, sar, sigB, sae, and arl, as well as by a number of proteins with homology to SarA. Rot (repressor of toxins), a SarA homologue, was previously identified in a library of transposon-induced mutants created in an agr-negative strain by screening for restored protease and alpha-toxin. To date, all of the SarA homologues have been shown to act as global regulators of virulence genes. Therefore, we investigated the extent of transcriptional regulation of staphylococcal genes by Rot. We compared the transcriptional profile of a rot agr double mutant to that of its agr parental strain by using custom-made Affymetrix GeneChips. Our findings indicate that Rot is not only a repressor but a global regulator with both positive and negative effects on the expression of S. aureus genes. Our data also indicate that Rot and agr have opposing effects on select target genes. These results provide further insight into the role of Rot in the regulatory cascade of S. aureus virulence gene expression.

* Corresponding author. Mailing address: Public Health Research Institute at the International Center of Public Health, Newark, NJ 07103. Phone: (973) 854-3240. Fax: (973) 854-3241. E-mail: barry{at}phri.org.

Journal of Bacteriology, January 2003, p. 610-619, Vol. 185, No. 2
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.2.610-619.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
Appl. Environ. Microbiol. Infect. Immun. Eukaryot. Cell
Mol. Cell. Biol. J. Virol. Microbiol. Mol. Biol. Rev.
ALL ASM JOURNALS

Copyright © 2003 by the American Society for Microbiology. All rights reserved.