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Formation of an F' Plasmid by Recombination between Imperfectly Repeated Chromosomal Rep Sequences: a Closer Look at an Old Friend (F'₁₂₈ pro lac)

Eric Kofoid, Ulfar Bergthorsson, E. Susan Slechta, and John R. Roth^*

Department of Biology, University of Utah, Salt Lake City, Utah 84122

Received 1 August 2002/ Accepted 28 October 2002

Plasmid F'₁₂₈ was formed by an exchange between chromosomal Rep sequences that placed lac near dinB between many pairs of Rep sequences. Plasmid F'₁₂₈ is critical for selection-enhanced lac reversion (adaptive mutation), which requires prior lac amplification. The structure of F'₁₂₈ supports the idea that amplification is initiated by Rep-Rep recombination and that general mutagenesis requires coamplification of dinB (error-prone polymerase) with lac.

Present address: Center for Genetics and Development, University of California, Davis, Davis, CA 95616.

Present address: Department of Biology, Indiana University, Bloomington, IN 47405.

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