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Journal of Bacteriology, January 2003, p. 664-668, Vol. 185, No. 2
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.2.664-668.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Flagella and Motility in Actinobacillus pleuropneumoniae

Erasmo Negrete-Abascal,1,4* Magda E. Reyes,2 Rosa M. García,3 Sergio Vaca,4 Jorge A. Girón,1 Octavio García,4 Edgar Zenteno,5 and Mireya de la Garza2

Centro de Investigaciones en Ciencias Microbiológicas, Benemérita Universidad Autónoma de Puebla, Puebla 72000,1 Departamento de Biología Celular,2 Departamento de Patología Experimental, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, México, D. F. 07000,3 Escuela de Biología, Facultad de Estudios Superiores IztacalaTlalnepantla, Edu. de México,4 Departamento de Bioquímica, Facultad de Medicina, Universidad Nacional Autónoma de México, México, D. F. 04510, Mexico5

Received 2 July 2002/ Accepted 15 October 2002

Actinobacillus pleuropneumoniae has been considered nonmotile and nonflagellate. In this work, it is demonstrated that A. pleuropneumoniae produces flagella composed of a 65-kDa protein with an N-terminal amino acid sequence that shows 100% identity with those of Escherichia coli, Salmonella, and Shigella flagellins. The DNA sequence obtained through PCR of the fliC gene in A. pleuropneumoniae showed considerable identity (93%) in its 5' and 3' ends with the DNA sequences of corresponding genes in E. coli, Salmonella enterica, and Shigella spp. The motility of A. pleuropneumoniae was observed in tryptic soy or brain heart infusion soft agar media, and it is influenced by temperature. Flagella and motility may be involved in the survival and pathogenesis of A. pleuropneumoniae in pigs.

* Corresponding author. Mailing address: Escuela de Biología, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Av. De los Barrios #1, Los Reyes Iztacala, Tlalnepantla, Edo. de México, C.P. 54090, Mexico. Phone: (52) 5556231254. Fax: (52) 5556231155. E-mail: negretee{at}yahoo.com.

Journal of Bacteriology, January 2003, p. 664-668, Vol. 185, No. 2
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.2.664-668.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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