This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sapunaric, F.
Right arrow Articles by Coyette, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sapunaric, F.
Right arrow Articles by Coyette, J.

 Previous Article  |  Next Article 

Journal of Bacteriology, October 2003, p. 5925-5935, Vol. 185, No. 20
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.20.5925-5935.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Redefining the Role of psr in ß-Lactam Resistance and Cell Autolysis of Enterococcus hirae

Frédéric Sapunaric,1 Christine Franssen,2 Patrick Stefanic,2 Ana Amoroso,3 Olivier Dardenne,4 and Jacques Coyette2*

Department of Molecular Biology and Microbiology, School of Medicine, Tufts University, Boston, Massachusetts 02111,1 Centre d'Ingénierie des Protéines, Université de Liège, Institut de Chimie, B-4000 Liège, Belgium,2 Laboratorio de Resistencia Bacteriana, Cátedra de Microbiologia, Facultad de Farmacia y Bioquimica, Universidad de Buenos Aires, Junin 956, 1113 Buenos Aires, Argentina,3 Genetics Unit, Shriners Hospital for Children, Montréal, Québec, Canada H3G 1A64

Received 3 March 2003/ Accepted 28 July 2003

The contribution of penicillin-binding protein 5 (PBP5) and the PBP5 synthesis repressor (Psr) to the ß-lactam resistance, growth, and cell autolysis of wild-type strain ATCC 9790 and resistant strain R40 of Enterococcus hirae was investigated by disruption or substitution of the corresponding pbp5 and psr genes by Campbell-type recombination. The resulting modifications were confirmed by hybridization and PCR. The low susceptibility of E. hirae to ß-lactams was confirmed to be largely dependent on the presence of PBP5. However, against all expectations, inactivation of psr in ATCC 9790 or complementation of R40 cells with psr did not modify the susceptibility to benzylpenicillin or the growth and cell autolysis rates. These results indicated that the psr gene does not seem to be involved in the regulation of PBP5 synthesis and consequently in ß-lactam resistance or in the regulation of cell autolysis in E. hirae.

* Corresponding author. Mailing address: Centre d'Ingénierie des Protéines, Université de Liège, Institut de Chimie, B6, Sart Tilman, B-4000 Liège, Belgium. Phone: (32) 4 366 33 99. Fax: (32) 4 366 33 64. E-mail: jcoyette{at}ulg.ac.be.

Journal of Bacteriology, October 2003, p. 5925-5935, Vol. 185, No. 20
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.20.5925-5935.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Johnsborg, O., Havarstein, L. S. (2009). Pneumococcal LytR, a Protein from the LytR-CpsA-Psr Family, Is Essential for Normal Septum Formation in Streptococcus pneumoniae. J. Bacteriol. 191: 5859-5864 [Abstract] [Full Text]  
  • Leimanis, S., Hoyez, N., Hubert, S., Laschet, M., Sauvage, E., Brasseur, R., Coyette, J. (2006). PBP5 Complementation of a PBP3 Deficiency in Enterococcus hirae.. J. Bacteriol. 188: 6298-6307 [Abstract] [Full Text]  
  • Chatfield, C. H., Koo, H., Quivey,, R. G. Jr (2005). The putative autolysin regulator LytR in Streptococcus mutans plays a role in cell division and is growth-phase regulated. Microbiology 151: 625-631 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»