This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Medina, G.
Right arrow Articles by Soberón-Chávez, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Medina, G.
Right arrow Articles by Soberón-Chávez, G.

 Previous Article  |  Next Article 

Journal of Bacteriology, October 2003, p. 5976-5983, Vol. 185, No. 20
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.20.5976-5983.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Mechanism of Pseudomonas aeruginosa RhlR Transcriptional Regulation of the rhlAB Promoter

Gerardo Medina,1 Katy Juárez,1 Brenda Valderrama,2 and Gloria Soberón-Chávez1*

Departamento de Microbiología Molecular,1 Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México2

Received 25 March 2003/ Accepted 25 July 2003

Pseudomonas aeruginosa contains two transcription regulators (LasR and RhlR) that, when complexed with their specific autoinducers (3-oxo-dodecanoyl-homoserine lactone and butanoyl-homoserine lactone, respectively) activate transcription of different virulence-associated traits. We studied the RhlR-dependent transcriptional regulation of the rhlAB operon encoding rhamnosyltransferase 1, an enzyme involved in the synthesis of the surfactant monorhamnolipid, and showed that RhlR binds to a specific sequence in the rhlAB regulatory region, both in the presence and in the absence of its autoinducer. Our data suggest that in the former case it activates transcription, whereas in the latter it acts as a transcriptional repressor of this promoter. RhlR seems to repress the transcription of other quorum-sensing-regulated genes; thus, RhlR repressor activity might be of importance in the finely regulated expression of P. aeruginosa virulence-associated traits.

* Corresponding author. Present address: Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, UNAM, Apdo. Postal 70-228, Ciudad de México 04510, México. Phone: (52) (55) 5622-3867. Fax: (52) (55) 5622-3855. E-mail: gloria{at}biomedicas.unam.mx.

Journal of Bacteriology, October 2003, p. 5976-5983, Vol. 185, No. 20
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.20.5976-5983.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Gupta, R., Gobble, T. R., Schuster, M. (2009). GidA Posttranscriptionally Regulates rhl Quorum Sensing in Pseudomonas aeruginosa. J. Bacteriol. 191: 5785-5792 [Abstract] [Full Text]  
  • Dekimpe, V., Deziel, E. (2009). Revisiting the quorum-sensing hierarchy in Pseudomonas aeruginosa: the transcriptional regulator RhlR regulates LasR-specific factors. Microbiology 155: 712-723 [Abstract] [Full Text]  
  • Yang, M., Giel, J. L., Cai, T., Zhong, Z., Zhu, J. (2009). The LuxR Family Quorum-Sensing Activator MrtR Requires Its Cognate Autoinducer for Dimerization and Activation but Not for Protein Folding. J. Bacteriol. 191: 434-438 [Abstract] [Full Text]  
  • Toyofuku, M., Nomura, N., Fujii, T., Takaya, N., Maseda, H., Sawada, I., Nakajima, T., Uchiyama, H. (2007). Quorum Sensing Regulates Denitrification in Pseudomonas aeruginosa PAO1. J. Bacteriol. 189: 4969-4972 [Abstract] [Full Text]  
  • Li, L.-L., Malone, J. E., Iglewski, B. H. (2007). Regulation of the Pseudomonas aeruginosa Quorum-Sensing Regulator VqsR. J. Bacteriol. 189: 4367-4374 [Abstract] [Full Text]  
  • Yan, A., Huang, X., Liu, H., Dong, D., Zhang, D., Zhang, X., Xu, Y. (2007). An rhl-like quorum-sensing system negatively regulates pyoluteorin production in Pseudomonas sp. M18. Microbiology 153: 16-28 [Abstract] [Full Text]  
  • Koch, B., Liljefors, T., Persson, T., Nielsen, J., Kjelleberg, S., Givskov, M. (2005). The LuxR receptor: the sites of interaction with quorum-sensing signals and inhibitors. Microbiology 151: 3589-3602 [Abstract] [Full Text]  
  • Lindsay, A., Ahmer, B. M. M. (2005). Effect of sdiA on Biosensors of N-Acylhomoserine Lactones. J. Bacteriol. 187: 5054-5058 [Abstract] [Full Text]  
  • Wade, D. S., Calfee, M. W., Rocha, E. R., Ling, E. A., Engstrom, E., Coleman, J. P., Pesci, E. C. (2005). Regulation of Pseudomonas Quinolone Signal Synthesis in Pseudomonas aeruginosa. J. Bacteriol. 187: 4372-4380 [Abstract] [Full Text]  
  • Lequette, Y., Greenberg, E. P. (2005). Timing and Localization of Rhamnolipid Synthesis Gene Expression in Pseudomonas aeruginosa Biofilms. J. Bacteriol. 187: 37-44 [Abstract] [Full Text]  
  • Ulrich, R. L., Hines, H. B., Parthasarathy, N., Jeddeloh, J. A. (2004). Mutational Analysis and Biochemical Characterization of the Burkholderia thailandensis DW503 Quorum-Sensing Network. J. Bacteriol. 186: 4350-4360 [Abstract] [Full Text]  
  • Heurlier, K., Williams, F., Heeb, S., Dormond, C., Pessi, G., Singer, D., Camara, M., Williams, P., Haas, D. (2004). Positive Control of Swarming, Rhamnolipid Synthesis, and Lipase Production by the Posttranscriptional RsmA/RsmZ System in Pseudomonas aeruginosa PAO1. J. Bacteriol. 186: 2936-2945 [Abstract] [Full Text]  
  • Williams, S. C., Patterson, E. K., Carty, N. L., Griswold, J. A., Hamood, A. N., Rumbaugh, K. P. (2004). Pseudomonas aeruginosa Autoinducer Enters and Functions in Mammalian Cells. J. Bacteriol. 186: 2281-2287 [Abstract] [Full Text]  
  • Medina, G., Juarez, K., Diaz, R., Soberon-Chavez, G. (2003). Transcriptional regulation of Pseudomonas aeruginosa rhlR, encoding a quorum-sensing regulatory protein. Microbiology 149: 3073-3081 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»