This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Caberoy, N. B.
Right arrow Articles by Garza, A. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Caberoy, N. B.
Right arrow Articles by Garza, A. G.

 Previous Article  |  Next Article 

Journal of Bacteriology, October 2003, p. 6083-6094, Vol. 185, No. 20
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.20.6083-6094.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Global Mutational Analysis of NtrC-Like Activators in Myxococcus xanthus: Identifying Activator Mutants Defective for Motility and Fruiting Body Development

Nora B. Caberoy,1 Roy D. Welch,2 Jimmy S. Jakobsen,2 Steven C. Slater,3 and Anthony G. Garza1*

School of Molecular Biosciences, Washington State University, Pullman, Washington 99164-4234,1 Department of Biochemistry, Stanford University School of Medicine, Stanford, California 94305-5329,2 Cereon Genomics, Monsanto Protein Technologies, Middleton, Wisconsin 53562-09993

Received 3 April 2003/ Accepted 18 July 2003

The multicellular developmental cycle of Myxococcus xanthus requires large-scale changes in gene transcription, and recent findings indicate that NtrC-like activators play a prominent role in regulating these changes. In this study, we made insertions in 28 uncharacterized ntrC-like activator (nla) genes and found that eight of these insertions cause developmental defects. Hence, these results are consistent with the idea that M. xanthus uses a series of different NtrC-like activators during fruiting body development. Four of the eight developmental mutants we identified have motility defects. The nla1, nla19, and nla23 mutants show S-motility defects, while the nla24 mutant shows defects in both S-motility and A-motility. During development, aggregation of the nla1, nla19, and nla23 mutants is delayed slightly and the nla24 mutant shows no signs of aggregation or sporulation. The nla4, nla6, nla18, and nla28 mutants have no appreciable loss in motility, but they fail to aggregate and to sporulate normally. The nla18 mutant belongs to a special class of developmental mutants whose defects can be rescued when they are codeveloped with wild-type cells, suggesting that nla18 fails to produce a cell-cell signal required for development. The three remaining activator mutants, nla4, nla6, and nla28, appear to have complex developmental phenotypes that include deficiencies in cell-cell developmental signals.

* Corresponding author. Mailing address: School of Molecular Biosciences, 301 Abelson Hall, Washington State University, Pullman, WA 99164-4234. Phone: (509) 335-7563. Fax: (509) 335-1907. E-mail: aggarza{at}wsu.edu.

Journal of Bacteriology, October 2003, p. 6083-6094, Vol. 185, No. 20
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.20.6083-6094.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Pan, H.-w., Liu, H., Liu, T., Li, C.-y., Li, Z.-f., Cai, K., Zhang, C.-y., Zhang, Y., Hu, W., Wu, Z.-h., Li, Y.-z. (2009). Seawater-Regulated Genes for Two-Component Systems and Outer Membrane Proteins in Myxococcus. J. Bacteriol. 191: 2102-2111 [Abstract] [Full Text]  
  • Taylor, R. G., Welch, R. D. (2008). Chemotaxis as an Emergent Property of a Swarm. J. Bacteriol. 190: 6811-6816 [Abstract] [Full Text]  
  • Whitworth, D. E., Cock, P. J. A. (2008). Two-component systems of the myxobacteria: structure, diversity and evolutionary relationships. Microbiology 154: 360-372 [Abstract] [Full Text]  
  • Ossa, F., Diodati, M. E., Caberoy, N. B., Giglio, K. M., Edmonds, M., Singer, M., Garza, A. G. (2007). The Myxococcus xanthus Nla4 Protein Is Important for Expression of Stringent Response-Associated Genes, ppGpp Accumulation, and Fruiting Body Development. J. Bacteriol. 189: 8474-8483 [Abstract] [Full Text]  
  • Youderian, P., Hartzell, P. L. (2007). Triple Mutants Uncover Three New Genes Required for Social Motility in Myxococcus xanthus. Genetics 177: 557-566 [Abstract] [Full Text]  
  • Dahl, J. L., Tengra, F. K., Dutton, D., Yan, J., Andacht, T. M., Coyne, L., Windell, V., Garza, A. G. (2007). Identification of Major Sporulation Proteins of Myxococcus xanthus Using a Proteomic Approach. J. Bacteriol. 189: 3187-3197 [Abstract] [Full Text]  
  • Tengra, F. K., Dahl, J. L., Dutton, D., Caberoy, N. B., Coyne, L., Garza, A. G. (2006). CbgA, a Protein Involved in Cortex Formation and Stress Resistance in Myxococcus xanthus Spores. J. Bacteriol. 188: 8299-8302 [Abstract] [Full Text]  
  • Velicer, G. J., Raddatz, G., Keller, H., Deiss, S., Lanz, C., Dinkelacker, I., Schuster, S. C. (2006). Comprehensive mutation identification in an evolved bacterial cooperator and its cheating ancestor. Proc. Natl. Acad. Sci. USA 103: 8107-8112 [Abstract] [Full Text]  
  • Diodati, M. E., Ossa, F., Caberoy, N. B., Jose, I. R., Hiraiwa, W., Igo, M. M., Singer, M., Garza, A. G. (2006). Nla18, a Key Regulatory Protein Required for Normal Growth and Development of Myxococcus xanthus.. J. Bacteriol. 188: 1733-1743 [Abstract] [Full Text]  
  • Youderian, P., Hartzell, P. L. (2006). Transposon Insertions of magellan-4 That Impair Social Gliding Motility in Myxococcus xanthus. Genetics 172: 1397-1410 [Abstract] [Full Text]  
  • Lancero, H. L., Castaneda, S., Caberoy, N. B., Ma, X., Garza, A. G., Shi, W. (2005). Analysing protein-protein interactions of the Myxococcus xanthus Dif signalling pathway using the yeast two-hybrid system. Microbiology 151: 1535-1541 [Abstract] [Full Text]  
  • Jelsbak, L., Kaiser, D. (2005). Regulating Pilin Expression Reveals a Threshold for S Motility in Myxococcus xanthus. J. Bacteriol. 187: 2105-2112 [Abstract] [Full Text]  
  • Kroos, L. (2005). Eukaryotic-like signaling and gene regulation in a prokaryote that undergoes multicellular development. Proc. Natl. Acad. Sci. USA 102: 2681-2682 [Full Text]  
  • Jelsbak, L., Givskov, M., Kaiser, D. (2005). Enhancer-binding proteins with a forkhead-associated domain and the {sigma}54 regulon in Myxococcus xanthus fruiting body development. Proc. Natl. Acad. Sci. USA 102: 3010-3015 [Abstract] [Full Text]  
  • Lancero, H., Caberoy, N. B., Castaneda, S., Li, Y., Lu, A., Dutton, D., Duan, X.-y., Kaplan, H. B., Shi, W., Garza, A. G. (2004). Characterization of a Myxococcus xanthus mutant that is defective for adventurous motility and social motility. Microbiology 150: 4085-4093 [Abstract] [Full Text]  
  • Jakobsen, J. S., Jelsbak, L., Jelsbak, L., Welch, R. D., Cummings, C., Goldman, B., Stark, E., Slater, S., Kaiser, D. (2004). {sigma}54 Enhancer Binding Proteins and Myxococcus xanthus Fruiting Body Development. J. Bacteriol. 186: 4361-4368 [Abstract] [Full Text]  
  • Nielsen, M., Rasmussen, A. Aa., Ellehauge, E., Treuner-Lange, A., Sogaard-Andersen, L. (2004). HthA, a putative DNA-binding protein, and HthB are important for fruiting body morphogenesis in Myxococcus xanthus. Microbiology 150: 2171-2183 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»