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Journal of Bacteriology, October 2003, p. 6192-6194, Vol. 185, No. 20
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.20.6192-6194.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Changes in the Concentrations of Guanosine 5'-Diphosphate 3'-Diphosphate and the Initiating Nucleoside Triphosphate Account for Inhibition of rRNA Transcription in Fructose-1,6-Diphosphate Aldolase (fda) Mutants

David A. Schneider and Richard L. Gourse*

Department of Bacteriology, University of Wisconsin, Madison, Wisconsin 53706

Received 23 June 2003/ Accepted 30 July 2003

Early screens for conditional lethal mutations that affected rRNA expression in Escherichia coli identified temperature-sensitive fda mutants (fda encodes the glycolytic enzyme fructose-1,6-diphosphate aldolase). It was shown that these fda(Ts) mutants were severely impaired in rRNA synthesis upon shift to the restrictive temperature, although the mechanism of inhibition was never determined. Here, we bring resolution to this long-standing question by showing that changes in the concentrations of guanosine 5'-diphosphate 3'-diphosphate and initiating nucleoside triphosphates can account for the previously observed effects of fda mutations on rRNA transcription.

* Corresponding author. Mailing address: Department of Bacteriology, University of Wisconsin, 420 Henry Mall, Madison, WI 53706. Phone: (608) 262-9813. Fax: (608) 262-9865. E-mail: rgourse{at}bact.wisc.edu.

Journal of Bacteriology, October 2003, p. 6192-6194, Vol. 185, No. 20
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.20.6192-6194.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Dennis, P. P., Ehrenberg, M., Bremer, H. (2004). Control of rRNA Synthesis in Escherichia coli: a Systems Biology Approach. Microbiol. Mol. Biol. Rev. 68: 639-668 [Abstract] [Full Text]  
  • Schneider, D. A., Gourse, R. L. (2004). Relationship between Growth Rate and ATP Concentration in Escherichia coli: A BIOASSAY FOR AVAILABLE CELLULAR ATP. J. Biol. Chem. 279: 8262-8268 [Abstract] [Full Text]  


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