This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Chang, W.-S. Articles by Halverson, L. J. Search for Related Content PubMed PubMed Citation Articles by Chang, W.-S. Articles by Halverson, L. J.

 Previous Article  |  Next Article 

Journal of Bacteriology, October 2003, p. 6199-6204, Vol. 185, No. 20
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.20.6199-6204.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Reduced Water Availability Influences the Dynamics, Development, and Ultrastructural Properties of Pseudomonas putida Biofilms

Department of Agronomy,² Graduate Program in Microbiology, Iowa State University, Ames, Iowa 50011-1010¹

Received 14 April 2003/ Accepted 19 July 2003

Pseudomonas putida strain mt-2 unsaturated biofilm formation proceeds through three distinct developmental phases, culminating in the formation of a microcolony. The form and severity of reduced water availability alter cell morphology, which influences microcolony size and ultrastructure. The dehydration (matric stress) treatments resulted in biofilms comprised of smaller cells, but they were taller and more porous and had a thicker extracellular polysaccharide layer at the air interface. In the solute stress treatments, cell filamentation occurred more frequently in the presence of high concentrations of ionic (but not nonionic) solutes, and these filamented cells drastically altered the biofilm architecture.

This article has been cited by other articles:

• Dechesne, A., Or, D., Gulez, G., Smets, B. F. (2008). The Porous Surface Model, a Novel Experimental System for Online Quantitative Observation of Microbial Processes under Unsaturated Conditions. Appl. Environ. Microbiol. 74: 5195-5200 [Abstract] [Full Text]  
• Chang, W.-S., van de Mortel, M., Nielsen, L., Nino de Guzman, G., Li, X., Halverson, L. J. (2007). Alginate Production by Pseudomonas putida Creates a Hydrated Microenvironment and Contributes to Biofilm Architecture and Stress Tolerance under Water-Limiting Conditions. J. Bacteriol. 189: 8290-8299 [Abstract] [Full Text]  
• Palmer, R. J. Jr., Stoodley, P. (2007). Biofilms 2007: Broadened Horizons and New Emphases. J. Bacteriol. 189: 7948-7960 [Full Text]  
• Jurcisek, J., Greiner, L., Watanabe, H., Zaleski, A., Apicella, M. A., Bakaletz, L. O. (2005). Role of Sialic Acid and Complex Carbohydrate Biosynthesis in Biofilm Formation by Nontypeable Haemophilus influenzae in the Chinchilla Middle Ear. Infect. Immun. 73: 3210-3218 [Abstract] [Full Text]  
• Danhorn, T., Hentzer, M., Givskov, M., Parsek, M. R., Fuqua, C. (2004). Phosphorus Limitation Enhances Biofilm Formation of the Plant Pathogen Agrobacterium tumefaciens through the PhoR-PhoB Regulatory System. J. Bacteriol. 186: 4492-4501 [Abstract] [Full Text]