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Characterization of divIVA and Other Genes Located in the Chromosomal Region Downstream of the dcw Cluster in Streptococcus pneumoniae

Dipartimento di Scienze Chirurgiche Sez. Microbiologia, Università di Cagliari, 09100 Cagliari,¹ GlaxoSmithKline Medicines Research Center, 37100 Verona, Italy²

Received 16 June 2003/ Accepted 14 July 2003

We analyzed the chromosome region of Streptococcus pneumoniae located downstream of the division and cell wall (dcw) cluster that contains the homolog of the Bacillus subtilis cell division gene divIVA and some genes of unknown function. Inactivation of divIVA in S. pneumoniae resulted in severe growth inhibition and defects in cell shape, nucleoid segregation, and cell division. Inactivation of the ylm genes resulted in some morphological and/or division abnormalities, depending on the inactivated gene. Transcriptional analysis revealed a relationship between these genes and the ftsA and ftsZ cell division genes, also indicating that the connection between the dcw cluster and the divIVA region is more extensive than just chromosomal position and gene organization.

This work is dedicated to the memory of Gerald D. Shockman, whose expertise and enthusiasm are greatly missed.

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