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Journal of Bacteriology, November 2003, p. 6463-6466, Vol. 185, No. 21
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.21.6463-6466.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Nucleotide Sequence of the Integration Site of the Temperate Bacteriophage 6220, Which Carries the Shiga Toxin Gene stx1ox3

Claudia Koch,1 Stefan Hertwig,2 and Bernd Appel2*

Schering AG, Biological Quality Control, D-13342 Berlin,1 Robert Koch-Institut, D-13353 Berlin, Germany2

Received 21 April 2003/ Accepted 31 July 2003

The integration site, attR, of the Shiga toxin-encoding phage 6220 (stx1ox3) has been determined. The phage integrates into the chromosome of its Escherichia coli host strain, CB6220, within a gene that is homologous to gene Z2577 and encodes an oxidoreductase. This new integration site was found in different Stx1ox3-producing enterohemorrhagic E. coli strains, which were analyzed by PCR.

* Corresponding author. Mailing address: (P12) Department of Biological Safety, Robert Koch-Institut, Nordufer 20, D-13353 Berlin, Germany. Phone: 49-30-4547-2100. Fax: 49-30-4547-2110. E-mail: appelb{at}rki.de.

Journal of Bacteriology, November 2003, p. 6463-6466, Vol. 185, No. 21
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.21.6463-6466.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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