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Thymine at —5 Is Crucial for cpc Promoter Activity of Synechocystis sp. Strain PCC 6714

School of Life Science, Tokyo University of Pharmacy and Life Science, Hachioji 192-0392,¹ CREST, Japan,² Institute of Molecular and Cellular Biosciences, University of Tokyo, Bunkyo-ku, Tokyo 113-0032,³ Computational Biology Research Center, National Institute of Advanced Industrial Science and Technology, Koutou-ku, Tokyo 135-0064,⁴ Advanced Technology Research Center, Mitsubishi Heavy Industries, Ltd., Kanazawa-ku, Yokohama 236-8515, Japan⁵

Received 1 May 2003/ Accepted 3 August 2003

The levels of transcripts of the cpc operon were highly reduced in a PD-1 mutant of cyanobacterium Synechocystis sp. strain PCC 6714. This was due to a substitution of C for T that occurred at 5 bp upstream of the transcription initiation site of the cpc operon. Any substitution for T at the -5 position drastically reduced both in vivo and in vitro promoter activity in cyanobacterium Synechococcus sp. strain PCC 7942 but not the in vivo activity in Escherichia coli. This suggests that the requirement of -5T appears to be specific for a cyanobacterial RNA polymerase-promoter combination.

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