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Journal of Bacteriology, November 2003, p. 6481-6485, Vol. 185, No. 21
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.21.6481-6485.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Bacteriophage ST64B, a Genetic Mosaic of Genes from Diverse Sources Isolated from Salmonella enterica Serovar Typhimurium DT 64

Princess T. Mmolawa,1,2,3 Horst Schmieger,4 and Michael W. Heuzenroeder1*

Infectious Diseases Laboratories, Institute of Medical and Veterinary Science, Adelaide, South Australia, 5000,1 Discipline of Microbiology and Immunology, Department of Molecular Biosciences, University of Adelaide, Adelaide, South Australia, 5005, Australia,2 Faculty of Agriculture, Science and Technology, Department of Animal Health, University of North West, Mmabatho, South Africa, 2735,3 Institute for Genetics and Microbiology, University of Munich, D-80638, Munich, Germany4

Received 10 March 2003/ Accepted 2 August 2003

The complete sequence of the double-stranded DNA (dsDNA) genome of the Salmonella enterica serovar Typhimurium ST64B bacteriophage was determined. The 40,149-bp genomic sequence of ST64B has an overall G+C content of 51.3% and is distinct from that of P22. The genome architecture is similar to that of the lambdoid phages, particularly that of coliphage {lambda}. Most of the putative tail genes showed sequence similarity to tail genes of Mu, a nonlambdoid phage. In addition, it is likely that these tail genes are not expressed due to insertions of fragments of genes related to virulence within some of the open reading frames. This, together with the inability of ST64B to produce plaques on a wide range of isolates, suggests that ST64B is a defective phage. In contrast to the tail genes, most of the head genes showed similarity to those of the lambdoid phages HK97 and HK022, but these head genes also have significant sequence similarities to those of several other dsDNA phages infecting diverse bacterial hosts, including Escherichia, Pseudomonas, Agrobacterium, Caulobacter, Mesorhizobium, and Streptomyces. This suggests that ST64B is a genetic mosaic that has acquired significant portions of its genome from sources outside the genus Salmonella.

* Corresponding author. Mailing address: P.O. Box 14, Rundle Mall, Adelaide, South Australia, 5000, Australia. Phone: 618 8222 3275. Fax: 618 8222 3543. E-mail: heuzenroeder{at}imvs.sa.gov.au.

Journal of Bacteriology, November 2003, p. 6481-6485, Vol. 185, No. 21
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.21.6481-6485.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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