This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mmolawa, P. T.
Right arrow Articles by Heuzenroeder, M. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mmolawa, P. T.
Right arrow Articles by Heuzenroeder, M. W.

 Previous Article  |  Next Article 

Journal of Bacteriology, November 2003, p. 6481-6485, Vol. 185, No. 21
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.21.6481-6485.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Bacteriophage ST64B, a Genetic Mosaic of Genes from Diverse Sources Isolated from Salmonella enterica Serovar Typhimurium DT 64

Princess T. Mmolawa,1,2,3 Horst Schmieger,4 and Michael W. Heuzenroeder1*

Infectious Diseases Laboratories, Institute of Medical and Veterinary Science, Adelaide, South Australia, 5000,1 Discipline of Microbiology and Immunology, Department of Molecular Biosciences, University of Adelaide, Adelaide, South Australia, 5005, Australia,2 Faculty of Agriculture, Science and Technology, Department of Animal Health, University of North West, Mmabatho, South Africa, 2735,3 Institute for Genetics and Microbiology, University of Munich, D-80638, Munich, Germany4

Received 10 March 2003/ Accepted 2 August 2003

The complete sequence of the double-stranded DNA (dsDNA) genome of the Salmonella enterica serovar Typhimurium ST64B bacteriophage was determined. The 40,149-bp genomic sequence of ST64B has an overall G+C content of 51.3% and is distinct from that of P22. The genome architecture is similar to that of the lambdoid phages, particularly that of coliphage {lambda}. Most of the putative tail genes showed sequence similarity to tail genes of Mu, a nonlambdoid phage. In addition, it is likely that these tail genes are not expressed due to insertions of fragments of genes related to virulence within some of the open reading frames. This, together with the inability of ST64B to produce plaques on a wide range of isolates, suggests that ST64B is a defective phage. In contrast to the tail genes, most of the head genes showed similarity to those of the lambdoid phages HK97 and HK022, but these head genes also have significant sequence similarities to those of several other dsDNA phages infecting diverse bacterial hosts, including Escherichia, Pseudomonas, Agrobacterium, Caulobacter, Mesorhizobium, and Streptomyces. This suggests that ST64B is a genetic mosaic that has acquired significant portions of its genome from sources outside the genus Salmonella.

* Corresponding author. Mailing address: P.O. Box 14, Rundle Mall, Adelaide, South Australia, 5000, Australia. Phone: 618 8222 3275. Fax: 618 8222 3543. E-mail: heuzenroeder{at}imvs.sa.gov.au.

Journal of Bacteriology, November 2003, p. 6481-6485, Vol. 185, No. 21
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.21.6481-6485.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Cooke, F. J., Brown, D. J., Fookes, M., Pickard, D., Ivens, A., Wain, J., Roberts, M., Kingsley, R. A., Thomson, N. R., Dougan, G. (2008). Characterization of the Genomes of a Diverse Collection of Salmonella enterica Serovar Typhimurium Definitive Phage Type 104. J. Bacteriol. 190: 8155-8162 [Abstract] [Full Text]  
  • Lima-Mendez, G., Van Helden, J., Toussaint, A., Leplae, R. (2008). Reticulate Representation of Evolutionary and Functional Relationships between Phage Genomes. Mol Biol Evol 25: 762-777 [Abstract] [Full Text]  
  • Kang, M.-S., Besser, T. E., Hancock, D. D., Porwollik, S., McClelland, M., Call, D. R. (2006). Identification of Specific Gene Sequences Conserved in Contemporary Epidemic Strains of Salmonella enterica. Appl. Environ. Microbiol. 72: 6938-6947 [Abstract] [Full Text]  
  • Hermans, A. P. H. M., Beuling, A. M., van Hoek, A. H. A. M., Aarts, H. J. M., Abee, T., Zwietering, M. H. (2006). Distribution of prophages and SGI-1 antibiotic-resistance genes among different Salmonella enterica serovar Typhimurium isolates. Microbiology 152: 2137-2147 [Abstract] [Full Text]  
  • Alonso, A., Pucciarelli, M. G., Figueroa-Bossi, N., Garcia-del Portillo, F. (2005). Increased Excision of the Salmonella Prophage ST64B Caused by a Deficiency in Dam Methylase. J. Bacteriol. 187: 7901-7911 [Abstract] [Full Text]  
  • Morales, C. A., Porwollik, S., Frye, J. G., Kinde, H., McClelland, M., Guard-Bouldin, J. (2005). Correlation of Phenotype with the Genotype of Egg-Contaminating Salmonella enterica Serovar Enteritidis. Appl. Environ. Microbiol. 71: 4388-4399 [Abstract] [Full Text]  
  • Kujat Choy, S. L., Boyle, E. C., Gal-Mor, O., Goode, D. L., Valdez, Y., Vallance, B. A., Finlay, B. B. (2004). SseK1 and SseK2 Are Novel Translocated Proteins of Salmonella enterica Serovar Typhimurium. Infect. Immun. 72: 5115-5125 [Abstract] [Full Text]  
  • Brussow, H., Canchaya, C., Hardt, W.-D. (2004). Phages and the Evolution of Bacterial Pathogens: from Genomic Rearrangements to Lysogenic Conversion. Microbiol. Mol. Biol. Rev. 68: 560-602 [Abstract] [Full Text]  
  • Figueroa-Bossi, N., Bossi, L. (2004). Resuscitation of a Defective Prophage in Salmonella Cocultures. J. Bacteriol. 186: 4038-4041 [Abstract] [Full Text]  
  • Goodwin, T. J. D., Poulter, R. T. M. (2004). A New Group of Tyrosine Recombinase-Encoding Retrotransposons. Mol Biol Evol 21: 746-759 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»