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Journal of Bacteriology, November 2003, p. 6556-6561, Vol. 185, No. 22
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.22.6556-6561.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Arginine-Agmatine Antiporter in Extreme Acid Resistance in Escherichia coli

Ram Iyer, Carole Williams, and Christopher Miller*

Department of Biochemistry, Howard Hughes Medical Institute, Brandeis University, Waltham, Massachusetts 02454

Received 9 June 2003/ Accepted 27 August 2003

The process of arginine-dependent extreme acid resistance (XAR) is one of several decarboxylase-antiporter systems that protects Escherichia coli and possibly other enteric bacteria from exposure to the strong acid environment of the stomach. Arginine-dependent acid resistance depends on an intracellular proton-utilizing arginine {alpha}-decarboxylase and a membrane transport protein necessary for delivering arginine to and removing agmatine, its decarboxylation product, from the cytoplasm. The arginine system afforded significant protection to wild-type E. coli cells in our acid shock experiments. The gene coding for the transport protein is identified here as a putative membrane protein of unknown function, YjdE, which we now name adiC. Strains from which this gene is deleted fail to mount arginine-dependent XAR, and they cannot perform coupled transport of arginine and agmatine. Homologues of this gene are found in other bacteria in close proximity to homologues of the arginine decarboxylase in a gene arrangement pattern similar to that in E coli. Evidence for a lysine-dependent XAR system in E. coli is also presented. The protection by lysine, however, is milder than that by arginine.

* Corresponding author. Mailing address: Department of Biochemistry, Howard Hughes Medical Institute, Brandeis University, Waltham, MA 02454. Phone: (781) 736-2340. Fax: (781) 736-2365. E-mail: cmiller{at}brandeis.edu.

Journal of Bacteriology, November 2003, p. 6556-6561, Vol. 185, No. 22
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.22.6556-6561.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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