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Journal of Bacteriology, November 2003, p. 6728-6731, Vol. 185, No. 22
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.22.6728-6731.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

In Vivo Effect of Mutations at the PRPP Binding Site of the Bacillus subtilis Purine Repressor

Pekka Rappu,^1* Terhi Pullinen,¹ and Pekka Mäntsälä¹

Department of Biochemistry and Food Chemistry, University of Turku, FIN-20014 Turku, Finland¹

Received 28 May 2003/ Accepted 18 August 2003

The Bacillus subtilis PurR mediates adenine repression and guanosine induction of purA. PRPP inhibits binding of PurR to DNA in vitro. Mutations in the PRPP binding motif of PurR caused strong repression regardless of purine exclusions or additions, establishing the role of PRPP as regulator of PurR.

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* Corresponding author. Mailing address: Department of Biochemistry and Food Chemistry, University of Turku, Vatselankatu 2, FIN-20014 Turku, Finland. Phone: 358-2 333 6856. Fax: 358-2 333 6860. E-mail: pekka.rappu{at}utu.fi.

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Journal of Bacteriology, November 2003, p. 6728-6731, Vol. 185, No. 22
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.22.6728-6731.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.