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Journal of Bacteriology, November 2003, p. 6741-6745, Vol. 185, No. 22
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.22.6741-6745.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Structure of Haloacetate-Catabolic IncP-1ß Plasmid pUO1 and Genetic Mobility of Its Residing Haloacetate-Catabolic Transposon

Department of Environmental Simulation, Institute for Environmental Sciences, Rokkasho, Aomori 039-3212,¹ Department of Environmental Life Sciences, Graduate School of Life Sciences, Tohoku University, Sendai 980-8577,² Department of Applied Biochemistry, Graduate School of Agriculture and Biological Science, Osaka Prefecture University, Sakai, Osaka 599-8531, Japan³

Received 6 June 2003/ Accepted 12 August 2003

The self-transmissible plasmid pUO1 from Delftia acidovorans strain B carries two haloacetate-catabolic transposons, TnHad1 and TnHad2, and the mer genes for resistance to mercury. The complete 67,066-bp sequence of pUO1 revealed that the mer genes were also carried by two Tn402/Tn5053-like transposons, Tn4671 and Tn4672, and that the pUO1 backbone regions shared 99% identity to those of the archetype IncP-1ß plasmid R751. Comparison of pUO1 with three other IncP-1ß plasmids illustrated the importance of transposon insertion in the diversity and evolution of this group of plasmids. Mutational analysis of the four outermost residues in the inverted repeats (IRs) of TnHad2, a Tn21-related transposon, revealed a crucial role of the second residue of its IRs in transposition.

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