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Journal of Bacteriology, December 2003, p. 7001-7007, Vol. 185, No. 23
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.23.7001-7007.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

The Quorum Sensing Negative Regulators EsaR and ExpR_Ecc, Homologues within the LuxR Family, Retain the Ability To Function as Activators of Transcription

Department of Plant Science, University of Connecticut, Storrs, Connecticut 06269,¹ Department of Biology, Virginia Tech, Blacksburg, Virginia 24061,² Department of Microbiology, University of Iowa, Iowa City, Iowa 52242³

Received 1 July 2003/ Accepted 10 September 2003

Most LuxR homologues function as activators of transcription during the process of quorum sensing, but a few, including EsaR and ExpR_Ecc, negatively impact gene expression. The LuxR-activated luxI promoter and LuxR binding site, the lux box, were used in artificial contexts to assess the potential for transcriptional activation and DNA binding by EsaR and ExpR_Ecc. Although the acyl-homoserine lactone responsiveness of both proteins is the opposite of that shown by most LuxR family members, EsaR and ExpR_Ecc have preserved the ability to interact with RNA polymerase and activate transcription despite their low affinity for the lux box DNA.

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