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Journal of Bacteriology, December 2003, p. 7008-7014, Vol. 185, No. 23
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.23.7008-7014.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Role of the Stationary Growth Phase Sigma Factor RpoS of Burkholderia pseudomallei in Response to Physiological Stress Conditions

Department of Biochemistry, Faculty of Science,¹ Department of Pathology, Faculty of Medicine- Ramathibodi Hospital, Mahidol University, Bangkok, Thailand,⁴ Division of Genomic Medicine, University of Sheffield Medical School, Sheffield, United Kingdom,² Institute of Molecular Biology and Genetics, Mahidol University, Nakorn Pathom, Thailand³

Received 17 March 2003/ Accepted 5 September 2003

The Burkholderia pseudomallei rpoS gene was identified, and an rpoS null mutant was constructed. The mutant was shown to have an increased sensitivity to carbon starvation and oxidative stress. By using rpoS-lacZ fusions, transcription of rpoS was shown to be growth phase regulated, reaching a peak upon entry into stationary phase.

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