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Journal of Bacteriology, December 2003, p. 7077-7084, Vol. 185, No. 24
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.24.7077-7084.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Development of a Physical and Genetic Map of the Virulent Wolbachia Strain wMelPop

Ling V. Sun,^1, Markus Riegler,² and Scott L. O'Neill^1,2*

Section of Vector Biology, Department of Epidemiology and Public Health, Yale University School of Medicine, New Haven, Connecticut 06520,¹ Department of Zoology and Entomology, School of Life Sciences, The University of Queensland, Brisbane, Queensland 4072, Australia²

Received 20 August 2003/ Accepted 26 September 2003

We report here the construction of a physical and genetic map of the virulent Wolbachia strain, wMelPop. This map was determined by ordering 28 chromosome fragments that resulted from digestion with the restriction endonucleases FseI, ApaI, SmaI, and AscI and were resolved by pulsed-field gel electrophoresis. Southern hybridization was done with 53 Wolbachia-specific genes as probes in order to determine the relative positions of these restriction fragments and use them to serve as markers. Comparison of the resulting map with the whole genome sequence of the closely related benign Wolbachia strain, wMel, shows that the two genomes are largely conserved in gene organization with the exception of a single inversion in the chromosome.

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* Corresponding author. Mailing address: Department of Zoology and Entomology, School of Life Sciences, The University of Queensland, Brisbane, QLD 4072, Australia. Phone: 61-7-3365-2471. Fax: 61-7-3346-9213. E-mail: scott.oneill{at}uq.edu.au.

Present address: Department of Genetics, Yale University, New Haven, CT 06520.

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Journal of Bacteriology, December 2003, p. 7077-7084, Vol. 185, No. 24
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.24.7077-7084.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

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