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Journal of Bacteriology, December 2003, p. 7085-7091, Vol. 185, No. 24
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.24.7085-7091.2003

Suppression of Factor-Dependent Transcription Termination by Antiterminator RNA

Rodney A. King{dagger} and Robert A. Weisberg*

Section on Microbial Genetics, Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892-2785

Received 13 June 2003/ Accepted 22 September 2003

Nascent transcripts of the phage HK022 put sites modify the transcription elongation complex so that it terminates less efficiently at intrinsic transcription terminators and accelerates through pause sites. We show here that the modification also suppresses termination in vivo at two factor-dependent terminators, one that depends on the bacterial Rho protein and a second that depends on the HK022-encoded Nun protein. Suppression was efficient when the termination factors were present at physiological levels, but an increase in the intracellular concentration of Nun increased termination both in the presence and absence of put. put-mediated antitermination thus shows no apparent terminator specificity, suggesting that put inhibits a step that is common to termination at the different types of terminator.

* Corresponding author. Mailing address: National Institute of Child Health and Human Development, NIH, Bldg. 6B, Room 3B308, Bethesda, MD 20892-2785. Phone: (301) 496-3555. Fax: (301) 496-0243. E-mail: rweisberg{at}nih.gov.

{dagger} Present address: Biology Department, Western Kentucky University, Bowling Green, KY 42101.

Journal of Bacteriology, December 2003, p. 7085-7091, Vol. 185, No. 24
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.24.7085-7091.2003






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