Journal of Bacteriology, December 2003, p. 7153-7159, Vol. 185, No. 24
0021-9193/03/$08.00+0 DOI: 10.1128/JB.185.24.7153-7159.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.
YmoA Negatively Regulates Expression of Invasin from Yersinia enterocolitica
Damon W. Ellison,1 Briana Young,1,
Kristin Nelson,1 and Virginia L. Miller1,2*
Departments
of Molecular Microbiology,1
Pediatrics,Washington University School of Medicine, St. Louis, Missouri
631102
Received 30 July 2003/
Accepted 23 September 2003
inv
encodes invasin, which is the primary invasion factor of Yersinia
enterocolitica. inv expression in vitro is regulated in
response to temperature, pH, and growth phase. In vitro, inv
is maximally expressed at 26°C and repressed at 37°C at
neutral pH but, when the pH of the media is adjusted to 5.5, levels of
inv expression at 37°C are comparable to those at
26°C. A previous genetic screen for regulators of inv
identified RovA, which was found to be required for activation of
inv in vitro under all conditions tested as well as in vivo.
Here we describe a screen that has identified a negative regulator of
inv expression, ymoA. The ymoBA locus was
identified by transposon mutagenesis as a repressor of inv
expression in vitro at 37°C at neutral pH. This mutant shows
increased inv expression at 37°C. The mutant can be
fully complemented for inv expression by a plasmid expressing
ymoA. These results indicate that YmoA plays a role in the
negative regulation of
inv.
* Corresponding
author. Mailing address: Washington University School of Medicine,
Department of Molecular Microbiology, 660 S. Euclid Ave., Campus Box
8230, St. Louis, MO 63110. Phone: (314) 286-2891. Fax: (314) 286-2896.
E-mail:
virginia{at}borcim.wustl.edu.
Present
address: Department of Food Science and Technology, University of
California, Davis, CA 95616.
Journal of Bacteriology, December 2003, p. 7153-7159, Vol. 185, No. 24
0021-9193/03/$08.00+0 DOI: 10.1128/JB.185.24.7153-7159.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.
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Copyright © 2003 by the American Society for Microbiology. All rights reserved.