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Journal of Bacteriology, December 2003, p. 7297-7300, Vol. 185, No. 24
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.24.7297-7300.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Control of Pseudomonas aeruginosa algZ Expression by the Alternative Sigma Factor AlgT

Daniel J. Wozniak,* April B. Sprinkle, and Patricia J. Baynham{dagger}

Department of Microbiology and Immunology, Wake Forest University School of Medicine, Winston-Salem, North Carolina 27157

Received 15 July 2003/ Accepted 23 September 2003

AlgZ controls Pseudomonas aeruginosa alginate synthesis by activating algD, yet algZ expression is not detectable in nonmucoid strains. Mobility shift and Western blot assays revealed that algZ expression requires the sigma factor AlgT. The mapped algZ transcription start site revealed a consensus AlgT-dependent promoter that, when mutated, substantially reduced algZ transcription.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, Wake Forest University School of Medicine, Medical Center Blvd., Winston-Salem, NC 27157-1064. Phone: (336) 716-2016. Fax: (336) 716-9928. E-mail: dwozniak{at}wfubmc.edu.

{dagger} Present address: Department of Biology, Thomas More College, Crestview Hills, Ky.

Journal of Bacteriology, December 2003, p. 7297-7300, Vol. 185, No. 24
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.24.7297-7300.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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