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Journal of Bacteriology, February 2003, p. 1097-1100, Vol. 185, No. 3
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.3.1097-1100.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Plasmid DNA Supercoiling and Gyrase Activity in Escherichia coli Wild-Type and rpoS Stationary-Phase Cells

Yazmid Reyes-Domínguez,{dagger} Gabriel Contreras-Ferrat, Jesús Ramírez-Santos, Jorge Membrillo-Hernández, and M. Carmen Gómez-Eichelmann*

Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, 04510 Mexico, Mexico

Received 12 August 2002/ Accepted 6 November 2002

Stationary-phase cells displayed a distribution of relaxed plasmids and had the ability to recover plasmid supercoiling as soon as nutrients became available. Preexisting gyrase molecules in these cells were responsible for this recovery. Stationary-phase rpoS cells showed a bimodal distribution of plasmids and failed to supercoil plasmids after the addition of nutrients, suggesting that rpoS plays a role in the regulation of plasmid topology during the stationary phase.

* Corresponding author. Mailing address: Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, UNAM, P.O. Box 70-228, 04510 Mexico, D.F., Mexico. Phone: (525) 556-22-38-52. Fax: (525) 556-22-38-55. E-mail: cargom{at}servidor.unam.mx.

{dagger} Present address: Institut de Génétique at Microbiologie, Université Paris-Sud, UMR, 8621 CNRS, 91405 Orsay Cedex, France.

Journal of Bacteriology, February 2003, p. 1097-1100, Vol. 185, No. 3
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.3.1097-1100.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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