This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Carmany, D. O.
Right arrow Articles by McCleary, W. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Carmany, D. O.
Right arrow Articles by McCleary, W. R.

 Previous Article  |  Next Article 

Journal of Bacteriology, February 2003, p. 1112-1115, Vol. 185, No. 3
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.3.1112-1115.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Genetic and Biochemical Studies of Phosphatase Activity of PhoR

Daniel O. Carmany,{dagger} Kristine Hollingsworth,{ddagger} and William R. McCleary*

Microbiology and Molecular Biology Department, Brigham Young University, Provo, Utah 84602-5253

Received 6 September 2002/ Accepted 30 October 2002

In Escherichia coli, PhoR is the histidine kinase of the phosphate regulon. It has been postulated that PhoR may function as a phospho-PhoB phosphatase. Experiments with four precise phoR deletion mutants supported this hypothesis and suggested that this activity resides within the histidine phosphorylation domain. This biochemical activity was confirmed by using a separately expressed histidine phosphorylation domain.

* Corresponding author. Mailing address: Microbiology and Molecular Biology Department, Brigham Young University, 775 WIDB, Provo, UT 84602-5253. Phone: (801) 422-8793. Fax: (801) 422-0519. E-mail: bill_mccleary{at}byu.edu.

{dagger} Present address: Life Science Division, Battelle Dugway Operations, Dugway Proving Ground, UT 84022.

{ddagger} Present address: Austin Community College, Austin, TX 78701.

Journal of Bacteriology, February 2003, p. 1112-1115, Vol. 185, No. 3
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.3.1112-1115.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Rice, C. D., Pollard, J. E., Lewis, Z. T., McCleary, W. R. (2009). Employment of a Promoter-Swapping Technique Shows that PhoU Modulates the Activity of the PstSCAB2 ABC Transporter in Escherichia coli. Appl. Environ. Microbiol. 75: 573-582 [Abstract] [Full Text]  
  • Porter, S. L., Roberts, M. A. J., Manning, C. S., Armitage, J. P. (2008). A bifunctional kinase-phosphatase in bacterial chemotaxis. Proc. Natl. Acad. Sci. USA 105: 18531-18536 [Abstract] [Full Text]  
  • Ferreira, G. M., Spira, B. (2008). The pst operon of enteropathogenic Escherichia coli enhances bacterial adherence to epithelial cells. Microbiology 154: 2025-2036 [Abstract] [Full Text]  
  • Shinar, G., Milo, R., Martinez, M. R., Alon, U. (2007). Input output robustness in simple bacterial signaling systems. Proc. Natl. Acad. Sci. USA 104: 19931-19935 [Abstract] [Full Text]  
  • Glover, R. T., Kriakov, J., Garforth, S. J., Baughn, A. D., Jacobs, W. R. Jr. (2007). The Two-Component Regulatory System senX3-regX3 Regulates Phosphate-Dependent Gene Expression in Mycobacterium smegmatis. J. Bacteriol. 189: 5495-5503 [Abstract] [Full Text]  
  • Wolfe, A. J. (2005). The Acetate Switch. Microbiol. Mol. Biol. Rev. 69: 12-50 [Abstract] [Full Text]  
  • Castelli, M. E., Cauerhff, A., Amongero, M., Soncini, F. C., Vescovi, E. G. (2003). The H Box-harboring Domain Is Key to the Function of the Salmonella enterica PhoQ Mg2+-sensor in the Recognition of Its Partner PhoP. J. Biol. Chem. 278: 23579-23585 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»