This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Maamar, H.
Right arrow Articles by Tardif, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Maamar, H.
Right arrow Articles by Tardif, C.

 Previous Article  |  Next Article 

Journal of Bacteriology, February 2003, p. 714-725, Vol. 185, No. 3
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.3.714-725.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

ISCce1 and ISCce2, Two Novel Insertion Sequences in Clostridium cellulolyticum

Hédia Maamar,1 Pascale de Philip,1,2 Jean-Pierre Bélaich,1,2 and Chantal Tardif1,2*

Laboratoire de Bioénergétique et Ingénierie des Protéines, UPR 9036-CNRS, 13402 Marseille Cedex 20,1 Université de Provence, 13331 Marseille Cedex 03, France2

Received 29 July 2002/ Accepted 4 November 2002

Two new insertion sequences, ISCce1 and ISCce2, were found to be inserted into the cipC gene of spontaneous mutants of Clostridium cellulolyticum. In these insertional mutants, the cipC gene was disrupted either by ISCce1 alone or by both ISCce1 and ISCce2. ISCce1 is 1,292 bp long and has one open reading frame. The open reading frame encodes a putative 348-amino-acid protein with significant levels of identity with putative proteins having unknown functions and with some transposases belonging to the IS481 and IS3 families. Imperfect 23-bp inverted repeats were found near the extremities of ISCce1. ISCce2 is 1,359 bp long, carries one open reading frame, and has imperfect 35-bp inverted repeats at its termini. The open reading frame encodes a putative 398-amino-acid protein. This protein shows significant levels of identity with transposases belonging to the IS256 family. Upon transposition, both ISCce1 and ISCce2 generate 8-bp direct repeats of the target sequence, but no consensus sequences could be identified at either insertion site. ISCce1 is copied at least 20 times in the genome, as assessed by Southern blot analysis. ISCce2 was found to be mostly inserted into ISCce1. In addition, as neither of the elements was detected in seven other Clostridium species, we concluded that they may be specific to the C. cellulolyticum strain used.

* Corresponding author. Mailing address: BIP-CNRS, 31 chemin Joseph Aiguier, 13402 Marseille Cedex 20, France. Phone: (33) 4 91 16 43 40. Fax: (33) 4 91 71 33 21. E-mail: tardif{at}ibsm.cnrs-mrs.fr.

Journal of Bacteriology, February 2003, p. 714-725, Vol. 185, No. 3
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.3.714-725.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Zverlov, V. V., Klupp, M., Krauss, J., Schwarz, W. H. (2008). Mutations in the Scaffoldin Gene, cipA, of Clostridium thermocellum with Impaired Cellulosome Formation and Cellulose Hydrolysis: Insertions of a New Transposable Element, IS1447, and Implications for Cellulase Synergism on Crystalline Cellulose. J. Bacteriol. 190: 4321-4327 [Abstract] [Full Text]  
  • Maamar, H., Abdou, L., Boileau, C., Valette, O., Tardif, C. (2006). Transcriptional Analysis of the cip-cel Gene Cluster from Clostridium cellulolyticum.. J. Bacteriol. 188: 2614-2624 [Abstract] [Full Text]  
  • Bayles, K. W., Welker, N. E., Winkler, M. E., Streips, U. N. (2003). Wind River Conference on Prokaryotic Biology--2002. J. Bacteriol. 185: 7-12 [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»