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Journal of Bacteriology, February 2003, p. 843-853, Vol. 185, No. 3
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.3.843-853.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

The Escherichia coli BarA-UvrY Two-Component System Is Needed for Efficient Switching between Glycolytic and Gluconeogenic Carbon Sources

Anna-Karin Pernestig,1 Dimitris Georgellis,2 Tony Romeo,3 Kazushi Suzuki,3 Henrik Tomenius,1 Staffan Normark,1 and Öjar Melefors1,4*

Microbiology and Tumorbiology Center, Karolinska Institutet, SE-17177 Stockholm,1 Swedish Institute for Infectious Disease Control, SE-17182 Solna, Sweden,4 Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, 04510 México D.F., Mexico,2 Department of Microbiology and Immunology, Emory University, Atlanta, Georgia 303223

Received 19 June 2002/ Accepted 5 November 2002

The Escherichia coli BarA and UvrY proteins were recently demonstrated to constitute a novel two-component system, although its function has remained largely elusive. Here we show that mutations in the sensor kinase gene, barA, or the response regulator gene, uvrY, in uropathogenic E. coli drastically affect survival in long-term competition cultures. Using media with gluconeogenic carbon sources, the mutants have a clear growth advantage when competing with the wild type, but using media with carbon sources feeding into the glycolysis leads to a clear growth advantage for the wild type. Results from competitions with mutants in the carbon storage regulation system, CsrA/B, known to be a master switch between glycolysis and gluconeogenesis, led us to propose that the BarA-UvrY two-component system controls the Csr system. Taking these results together, we propose the BarA-UvrY two-component system is crucial for efficient adaptation between different metabolic pathways, an essential function for adaptation to a new environment.

* Corresponding author. Mailing address: Microbiology and Tumorbiology Center, Karolinska Institutet, SE-17177 Stockholm, Sweden. Phone: 46-84572414. Fax: 46-8302566. E-mail: ojar.melefors{at}mtc.ki.se.

Journal of Bacteriology, February 2003, p. 843-853, Vol. 185, No. 3
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.3.843-853.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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