This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Otsuka, Y. Articles by Yonesaki, T. Search for Related Content PubMed PubMed Citation Articles by Otsuka, Y. Articles by Yonesaki, T.

Escherichia coli Endoribonucleases Involved in Cleavage of Bacteriophage T4 mRNAs

Yuichi Otsuka, Hiroyuki Ueno, and Tetsuro Yonesaki^*

Department of Biology, Graduate School of Science, Osaka University, Osaka 560-0043, Japan

Received 8 August 2002/ Accepted 11 November 2002

The dmd mutant of bacteriophage T4 has a defect in growth because of rapid degradation of late-gene mRNAs, presumably caused by mutant-specific cleavages of RNA. Some such cleavages can occur in an allele-specific manner, depending on the translatability of RNA or the presence of a termination codon. Other cleavages are independent of translation. In the present study, by introducing plasmids carrying various soc alleles, we could detect cleavages of soc RNA in uninfected cells identical to those found in dmd mutant-infected cells. We isolated five Escherichia coli mutant strains in which the dmd mutant was able to grow. One of these strains completely suppressed the dmd mutant-specific cleavages of soc RNA. The loci of the E. coli mutations and the effects of mutations in known RNase-encoding genes suggested that an RNA cleavage activity causing the dmd mutant-specific mRNA degradation is attributable to a novel RNase. In addition, we present evidence that 5'-truncated soc RNA, a stable form in T4-infected cells regardless of the presence of a dmd mutation, is generated by RNase E.

Present address: R&D Laboratories, Nippon Organon K.K., Osaka, Japan.

This article has been cited by other articles:

• Yamanishi, H., Yonesaki, T. (2005). RNA Cleavage Linked With Ribosomal Action. Genetics 171: 419-425 [Abstract] [Full Text]  
• Otsuka, Y., Yonesaki, T. (2005). A Novel Endoribonuclease, RNase LS, in Escherichia coli. Genetics 169: 13-20 [Abstract] [Full Text]  
• Piesiniene, L., Truncaite, L., Zajanckauskaite, A., Nivinskas, R. (2004). The sequences and activities of RegB endoribonucleases of T4-related bacteriophages. Nucleic Acids Res 32: 5582-5595 [Abstract] [Full Text]  
• Wei, X., Sayavedra-Soto, L. A., Arp, D. J. (2004). The transcription of the cbb operon in Nitrosomonas europaea. Microbiology 150: 1869-1879 [Abstract] [Full Text]