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Journal of Bacteriology, March 2003, p. 1672-1680, Vol. 185, No. 5
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.5.1672-1680.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Complex Regulation of the Bacillus subtilis Aconitase Gene

Hyun-Jin Kim,1 Sam-In Kim,1 Manoja Ratnayake-Lecamwasam,1,{dagger} Kiyoshi Tachikawa,1,{ddagger} Abraham L. Sonenshein,1* and Mark Strauch2

Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts 02111,1 Department of Oral and Craniofacial Biological Sciences, University of Maryland Dental School, Baltimore, Maryland 212012

Received 18 October 2002/ Accepted 10 December 2002

The roles of the CcpC, CodY, and AbrB proteins in regulation of the Bacillus subtilis aconitase (citB) gene were found to be distinct and to vary with the conditions and phase of growth. CcpC, a citrate-inhibited repressor that is the primary factor regulating citB expression in minimal-glucose-glutamine medium, also contributed to repression of citB during exponential-phase growth in broth medium. A null mutation in codY had no effect on citB expression during growth in minimal medium even when combined with ccpC and abrB mutations. However, a codY mutation slightly relieved repression during exponential growth in broth medium and completely derepressed citB expression when combined with a ccpC mutation. An abrB mutation led to decreased expression of citB during stationary phase in both broth and minimal medium. All three proteins bound in vitro to specific and partially overlapping sites within the citB regulatory region. Interaction of CcpC and CodY with the citB promoter region was partially competitive.

* Corresponding author. Mailing address: Department of Molecular Biology and Microbiology, Tufts University School of Medicine, 136 Harrison Avenue, Boston, MA 02111. Phone: (617) 636-6761. Fax: (617) 636-0337. E-mail: linc.sonenshein{at}tufts.edu.

{dagger} Present address: Corporate-Sponsored Research and Licensing, Massachusetts General Hospital, Charlestown, MA 02129.

{ddagger} Present address: Torrey Mesa Research Institute, Syngenta, La Jolla, CA 92121.

Journal of Bacteriology, March 2003, p. 1672-1680, Vol. 185, No. 5
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.5.1672-1680.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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