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Journal of Bacteriology, March 2003, p. 1719-1725, Vol. 185, No. 5
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.5.1719-1725.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

DNA Microarray Analysis of Redox-Responsive Genes in the Genome of the Cyanobacterium Synechocystis sp. Strain PCC 6803

Yukako Hihara,1* Kintake Sonoike,2 Minoru Kanehisa,3 and Masahiko Ikeuchi4

Department of Biochemistry and Molecular Biology, Faculty of Science, Saitama University, Saitama-shi, Saitama 338-8570,1 Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Kashiwanoha 5-1-5, Kashiwa, Chiba 277-8562,2 Institute for Chemical Research, Kyoto University, Uji, Kyoto 611-0011,3 Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, Tokyo 153-8902, Japan4

Received 10 September 2002/ Accepted 13 December 2002

Whole-genome DNA microarrays were used to evaluate the effect of the redox state of the photosynthetic electron transport chain on gene expression in Synechocystis sp. strain PCC 6803. Two specific inhibitors of electron transport, 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) and 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone (DBMIB), were added to the cultures, and changes in accumulation of transcripts were examined. About 140 genes were highlighted as reproducibly affected by the change in the redox state of the photosynthetic electron transport chain. It was shown that some stress-responsive genes but not photosynthetic genes were under the control of the redox state of the plastoquinone pool in Synechocystis sp. strain PCC 6803.

* Corresponding author. Mailing address: Department of Biochemistry and Molecular Biology, Faculty of Science, Saitama University, Saitama-shi, Saitama 338-8570, Japan. Phone: 81-48-858-3396. Fax: 81-48-858-3384. E-mail: hihara{at}molbiol.saitama-u.ac.jp.

Journal of Bacteriology, March 2003, p. 1719-1725, Vol. 185, No. 5
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.5.1719-1725.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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