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Journal of Bacteriology, March 2003, p. 1730-1733, Vol. 185, No. 5
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.5.1730-1733.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Mechanism of cis-trans Isomerization of Unsaturated Fatty Acids in Pseudomonas putida

Angelika von Wallbrunn,1 Hans Hermann Richnow,2 Grit Neumann,2 Friedhelm Meinhardt,1 and Hermann J. Heipieper2*

Institut für Mikrobiologie, Westfälische Wilhelms-Universität Münster, 48149 Münster,1 Department of Remediation Research, Centre for Environmental Research (UFZ) Leipzig-Halle, 04318 Leipzig, Germany2

Received 11 September 2002/ Accepted 28 November 2002

We studied the pattern of the cis-trans isomerization of unsaturated fatty acids in cells of Pseudomonas putida S12 grown in a medium supplemented with oleic acid which was deuterated at both of the C atoms of its double bond. Direct evidence that isomerization does not include a transient saturation of the double bond was obtained. In addition, analysis of the amino acid sequences of the seven known Cti proteins identified them as heme-containing proteins of the cytochrome c type.


* Corresponding author. Mailing address: Department of Remediation Research, Centre for Environmental Research (UFZ) Leipzig-Halle, Permoserstr. 15, 04318 Leipzig, Germany. Phone: 49 341 235 2772. Fax: 49 341 235 2492. E-mail: heipiep{at}san.ufz.de.


Journal of Bacteriology, March 2003, p. 1730-1733, Vol. 185, No. 5
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.5.1730-1733.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.




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