Journal of Bacteriology, March 2003, p. 1730-1733, Vol. 185, No. 5
0021-9193/03/$08.00+0 DOI: 10.1128/JB.185.5.1730-1733.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.
Mechanism of cis-trans Isomerization of Unsaturated Fatty Acids in Pseudomonas putida
Angelika von Wallbrunn,1 Hans Hermann Richnow,2 Grit Neumann,2 Friedhelm Meinhardt,1 and Hermann J. Heipieper2*
Institut für Mikrobiologie, Westfälische Wilhelms-Universität Münster, 48149 Münster,1
Department of Remediation Research, Centre for Environmental Research (UFZ) Leipzig-Halle, 04318 Leipzig, Germany2
Received 11 September 2002/
Accepted 28 November 2002
We studied the pattern of the cis-trans isomerization of unsaturated fatty acids in cells of Pseudomonas putida S12 grown in a medium supplemented with oleic acid which was deuterated at both of the C atoms of its double bond. Direct evidence that isomerization does not include a transient saturation of the double bond was obtained. In addition, analysis of the amino acid sequences of the seven known Cti proteins identified them as heme-containing proteins of the cytochrome c type.
* Corresponding author. Mailing address: Department of Remediation Research, Centre for Environmental Research (UFZ) Leipzig-Halle, Permoserstr. 15, 04318 Leipzig, Germany. Phone: 49 341 235 2772. Fax: 49 341 235 2492. E-mail: heipiep{at}san.ufz.de.
Journal of Bacteriology, March 2003, p. 1730-1733, Vol. 185, No. 5
0021-9193/03/$08.00+0 DOI: 10.1128/JB.185.5.1730-1733.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.
This article has been cited by other articles:
-
Goldberg, J. B., Hancock, R. E. W., Parales, R. E., Loper, J., Cornelis, P.
(2008). Pseudomonas 2007. J. Bacteriol.
190: 2649-2662
[Full Text]
-
Dominguez-Cuevas, P., Gonzalez-Pastor, J.-E., Marques, S., Ramos, J.-L., de Lorenzo, V.
(2006). Transcriptional Tradeoff between Metabolic and Stress-response Programs in Pseudomonas putida KT2440 Cells Exposed to Toluene. J. Biol. Chem.
281: 11981-11991
[Abstract]
[Full Text]
-
Neumann, G., Teras, R., Monson, L., Kivisaar, M., Schauer, F., Heipieper, H. J.
(2004). Simultaneous Degradation of Atrazine and Phenol by Pseudomonas sp. Strain ADP: Effects of Toxicity and Adaptation. Appl. Environ. Microbiol.
70: 1907-1912
[Abstract]
[Full Text]
-
Chiou, R. Y.-Y., Phillips, R. D., Zhao, P., Doyle, M. P., Beuchat, L. R.
(2004). Ethanol-Mediated Variations in Cellular Fatty Acid Composition and Protein Profiles of Two Genotypically Different Strains of Escherichia coli O157:H7. Appl. Environ. Microbiol.
70: 2204-2210
[Abstract]
[Full Text]
Copyright © 2003 by the American Society for Microbiology. All rights reserved.