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Journal of Bacteriology, March 2003, p. 1739-1744, Vol. 185, No. 5
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.5.1739-1744.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Hemin Binding, Functional Expression, and Complementation Analysis of Pap 31 from Bartonella henselae

Rainer Zimmermann,1 Volkhard A. J. Kempf,2 Emile Schiltz,3 Karin Oberle,1 and Anna Sander1*

Institute of Medical Microbiology and Hygiene,1 Institute of Organic Chemistry and Biochemistry, University of Freiburg, Freiburg,3 Institute of Microbiology, University of Tübingen, Tübingen, Germany2

Received 19 September 2002/ Accepted 28 November 2002

Growth of Bartonella henselae is strongly heme dependent, and B. henselae is unable to synthesize heme itself. At least five outer membrane-associated proteins from B. henselae bind hemin, including the 31-kDa protein designated Pap31. The gene of this protein was heterologously expressed in Escherichia coli M15(pREP4) and detected with monoclonal antibodies in the outer membrane fraction. Complementation of the hemA-deficient mutant E. coli K-12 EB53 (aroB tsx malT hemA) with pap31 demonstrated that this protein is involved in heme acquisition and may be an important virulence factor in the pathogenesis of B. henselae.

* Corresponding author. Mailing address: Institute of Medical Microbiology and Hygiene, University of Freiburg, Hermann-Herder Str. 11, D-79104 Freiburg, Germany. Phone: 49-761/203-6529. Fax: 49-761/203-6562. E-mail: sander{at}ukl.uni-freiburg.de.

Journal of Bacteriology, March 2003, p. 1739-1744, Vol. 185, No. 5
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.5.1739-1744.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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