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Journal of Bacteriology, March 2003, p. 1825-1830, Vol. 185, No. 6
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.6.1825-1830.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

tmRNA in Caulobacter crescentus Is Cell Cycle Regulated by Temporally Controlled Transcription and RNA Degradation

Kenneth C. Keiler1* and Lucy Shapiro2

Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania 16802,1 Department of Developmental Biology, Stanford University, Stanford, California 943052

Received 11 September 2002/ Accepted 16 December 2002

SsrA, or tmRNA, is a small RNA found in all bacteria that intervenes in selected translation reactions to target the nascent polypeptide for rapid proteolysis. We have found that the abundance of SsrA RNA in Caulobacter crescentus is regulated with respect to the cell cycle. SsrA RNA abundance increases in late G1 phase, peaks during the G1-S transition, and declines in early S phase, in keeping with the reported role for SsrA in the timing of DNA replication initiation. Cell cycle regulation of SsrA RNA is accomplished by a combination of temporally controlled transcription and regulated RNA degradation. Transcription from the ssrA promoter peaks late in G1, just before the peak in SsrA RNA abundance. SsrA RNA is stable in G1-phase cells and late S-phase cells but is degraded with a half-life of 4 to 5 min at the onset of S phase. This degradation is surprising, since SsrA RNA is both highly structured and highly abundant. This is the first observation of a structural RNA that is cell cycle regulated.

* Corresponding author. Mailing address: Department of Biochemistry and Molecular Biology, The Pennsylvania State University, 266 N. Frear Laboratory, University Park, PA 16802. Phone: (814) 863-0787. Fax: (814) 863-7024. E-mail: kkeiler{at}psu.edu.

Journal of Bacteriology, March 2003, p. 1825-1830, Vol. 185, No. 6
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.6.1825-1830.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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