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Journal of Bacteriology, March 2003, p. 1911-1922, Vol. 185, No. 6
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.6.1911-1922.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Additional Targets of the Bacillus subtilis Global Regulator CodY Identified by Chromatin Immunoprecipitation and Genome-Wide Transcript Analysis

Department of Cellular and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138,¹ Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts 02111,³ Faculty of Engineering, Fukuyama University, Fukuyama, Japan²

Received 19 November 2002/ Accepted 30 December 2002

Additional targets of CodY, a GTP-activated repressor of early stationary-phase genes in Bacillus subtilis, were identified by combining chromatin immunoprecipitation, DNA microarray hybridization, and gel mobility shift assays. The direct targets of CodY newly identified by this approach included regulatory genes for sporulation, genes that are likely to encode transporters for amino acids and sugars, and the genes for biosynthesis of branched-chain amino acids.

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