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Journal of Bacteriology, March 2003, p. 2022-2025, Vol. 185, No. 6
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.6.2022-2025.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Plasmid R16 ArdA Protein Preferentially Targets Restriction Activity of the Type I Restriction-Modification System EcoKI

Angela T. Thomas,1 William J. Brammar,2 and Brian M. Wilkins1*

Department of Genetics,1 Department of Biochemistry, University of Leicester, Leicester LE1 7RH, United Kingdom2

Received 23 September 2002/ Accepted 16 December 2002

The ArdA antirestriction protein of the IncB plasmid R16 selectively inhibited the restriction activity of EcoKI, leaving significant levels of modification activity under conditions in which restriction was almost completely prevented. The results are consistent with the hypothesis that ArdA functions in bacterial conjugation to allow an unmodified plasmid to evade restriction in the recipient bacterium and yet acquire cognate modification.

* Corresponding author. Mailing address: Department of Genetics, University of Leicester, Leicester LE1 7RH, United Kingdom. Phone: 44 (0) 116 252 3438. Fax: 44 (0) 116 252 5101. E-mail: bmw1{at}le.ac.uk.

Journal of Bacteriology, March 2003, p. 2022-2025, Vol. 185, No. 6
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.6.2022-2025.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • McMahon, S. A., Roberts, G. A., Johnson, K. A., Cooper, L. P., Liu, H., White, J. H., Carter, L. G., Sanghvi, B., Oke, M., Walkinshaw, M. D., Blakely, G. W., Naismith, J. H., Dryden, D. T. F. (2009). Extensive DNA mimicry by the ArdA anti-restriction protein and its role in the spread of antibiotic resistance. Nucleic Acids Res 0: gkp478v1-gkp478 [Abstract] [Full Text]  
  • Loenen, W. A. M. (2003). Tracking EcoKI and DNA fifty years on: a golden story full of surprises. Nucleic Acids Res 31: 7059-7069 [Abstract] [Full Text]  


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