This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Chan, P. F. Articles by Zalacain, M. Search for Related Content PubMed PubMed Citation Articles by Chan, P. F. Articles by Zalacain, M.

Characterization of a Novel Fucose-Regulated Promoter (P_fcsK) Suitable for Gene Essentiality and Antibacterial Mode-of-Action Studies in Streptococcus pneumoniae

Pan F. Chan,^* Karen M. O'Dwyer, Leslie M. Palmer, Jennifer D. Ambrad, Karen A. Ingraham, Chi So, Michael A. Lonetto, Sanjoy Biswas, Martin Rosenberg, David J. Holmes, and Magdalena Zalacain

Microbial, Musculoskeletal and Proliferative Diseases Center of Excellence for Drug Discovery, GlaxoSmithKline Pharmaceuticals, Collegeville, Pennsylvania 19426

Received 11 September 2002/ Accepted 18 December 2002

The promoter of the Streptococcus pneumoniae putative fuculose kinase gene (fcsK), the first gene of a novel fucose utilization operon, is induced by fucose and repressed by glucose or sucrose. When the streptococcal polypeptide deformylase (PDF) gene (def1, encoding PDF) was placed under the control of P_fcsK, fucose-dependent growth of the S. pneumoniae (P_fcsK::def1) strain was observed, confirming the essential nature of PDF in this organism. The mode of antibacterial action of actinonin, a known PDF inhibitor, was also confirmed with this strain. The endogenous fuculose kinase promoter is a tightly regulated, titratable promoter which will be useful for target validation and for confirmation of the mode of action of novel antibacterial drugs in S. pneumoniae.

Present address: Promega Corporation, Madison, WI 53711.

This article has been cited by other articles:

• Le Gouellec, A., Roux, L., Fadda, D., Massidda, O., Vernet, T., Zapun, A. (2008). Roles of Pneumococcal DivIB in Cell Division. J. Bacteriol. 190: 4501-4511 [Abstract] [Full Text]  
• Boraston, A. B., Wang, D., Burke, R. D. (2006). Blood Group Antigen Recognition by a Streptococcus pneumoniae Virulence Factor. J. Biol. Chem. 281: 35263-35271 [Abstract] [Full Text]  
• Teo, J. W. P., Thayalan, P., Beer, D., Yap, A. S. L., Nanjundappa, M., Ngew, X., Duraiswamy, J., Liung, S., Dartois, V., Schreiber, M., Hasan, S., Cynamon, M., Ryder, N. S., Yang, X., Weidmann, B., Bracken, K., Dick, T., Mukherjee, K. (2006). Peptide Deformylase Inhibitors as Potent Antimycobacterial Agents. Antimicrob. Agents Chemother. 50: 3665-3673 [Abstract] [Full Text]  
• Huang, J., Van Aller, G. S., Taylor, A. N., Kerrigan, J. J., Liu, W.-S., Trulli, J. M., Lai, Z., Holmes, D., Aubart, K. M., Brown, J. R., Zalacain, M. (2006). Phylogenomic and Biochemical Characterization of Three Legionella pneumophila Polypeptide Deformylases.. J. Bacteriol. 188: 5249-5257 [Abstract] [Full Text]  
• Guiral, S., Henard, V., Laaberki, M.-H., Granadel, C., Prudhomme, M., Martin, B., Claverys, J.-P. (2006). Construction and evaluation of a chromosomal expression platform (CEP) for ectopic, maltose-driven gene expression in Streptococcus pneumoniae. Microbiology 152: 343-349 [Abstract] [Full Text]  
• Kloosterman, T. G., Bijlsma, J. J. E., Kok, J., Kuipers, O. P. (2006). To have neighbour's fare: extending the molecular toolbox for Streptococcus pneumoniae. Microbiology 152: 351-359 [Abstract] [Full Text]  
• Odom, E. W., Vasta, G. R. (2006). Characterization of a Binary Tandem Domain F-type Lectin from Striped Bass (Morone saxatilis). J. Biol. Chem. 281: 1698-1713 [Abstract] [Full Text]  
• Ng, W.-L., Tsui, H.-C. T., Winkler, M. E. (2005). Regulation of the pspA Virulence Factor and Essential pcsB Murein Biosynthetic Genes by the Phosphorylated VicR (YycF) Response Regulator in Streptococcus pneumoniae. J. Bacteriol. 187: 7444-7459 [Abstract] [Full Text]  
• Zuniga, M., Comas, I., Linaje, R., Monedero, V., Yebra, M. J., Esteban, C. D., Deutscher, J., Perez-Martinez, G., Gonzalez-Candelas, F. (2005). Horizontal Gene Transfer in the Molecular Evolution of Mannose PTS Transporters. Mol Biol Evol 22: 1673-1685 [Abstract] [Full Text]  
• Carroll, P., Muttucumaru, D. G. N., Parish, T. (2005). Use of a Tetracycline-Inducible System for Conditional Expression in Mycobacterium tuberculosis and Mycobacterium smegmatis. Appl. Environ. Microbiol. 71: 3077-3084 [Abstract] [Full Text]  
• Haas, W., Sublett, J., Kaushal, D., Tuomanen, E. I. (2004). Revising the Role of the Pneumococcal vex-vncRS Locus in Vancomycin Tolerance. J. Bacteriol. 186: 8463-8471 [Abstract] [Full Text]  
• O'Dwyer, K., Watts, J. M., Biswas, S., Ambrad, J., Barber, M., Brule, H., Petit, C., Holmes, D. J., Zalacain, M., Holmes, W. M. (2004). Characterization of Streptococcus pneumoniae TrmD, a tRNA Methyltransferase Essential for Growth. J. Bacteriol. 186: 2346-2354 [Abstract] [Full Text]  
• Robertson, G. T., Ng, W.-L., Gilmour, R., Winkler, M. E. (2003). Essentiality of clpX, but Not clpP, clpL, clpC, or clpE, in Streptococcus pneumoniae R6. J. Bacteriol. 185: 2961-2966 [Abstract] [Full Text]