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Journal of Bacteriology, April 2003, p. 2080-2095, Vol. 185, No. 7
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.7.2080-2095.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Microarray Analysis of Pseudomonas aeruginosa Quorum-Sensing Regulons: Effects of Growth Phase and Environment{dagger}

Victoria E. Wagner,1 Daniel Bushnell,1 Luciano Passador,1 Andrew I. Brooks,2 and Barbara H. Iglewski1*

Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry,1 Department of Environmental Medicine and Center for Functional Genomics, University of Rochester Medical Center, Rochester, New York 146422

Received 6 September 2002/ Accepted 30 December 2002

Bacterial communication via quorum sensing (QS) has been reported to be important in the production of virulence factors, antibiotic sensitivity, and biofilm development. Two QS systems, known as the las and rhl systems, have been identified previously in the opportunistic pathogen Pseudomonas aeruginosa. High-density oligonucleotide microarrays for the P. aeruginosa PAO1 genome were used to investigate global gene expression patterns modulated by QS regulons. In the initial experiments we focused on identifying las and/or rhl QS-regulated genes using a QS signal generation-deficient mutant (PAO-JP2) that was cultured with and without added exogenous autoinducers [N-(3-oxododecanoyl) homoserine lactone and N-butyryl homoserine lactone]. Conservatively, 616 genes showed statistically significant differential expression (P <= 0.05) in response to the exogenous autoinducers and were classified as QS regulated. A total of 244 genes were identified as being QS regulated at the mid-logarithmic phase, and 450 genes were identified as being QS regulated at the early stationary phase. Most of the previously reported QS-promoted genes were confirmed, and a large number of additional QS-promoted genes were identified. Importantly, 222 genes were identified as being QS repressed. Environmental factors, such as medium composition and oxygen availability, eliminated detection of transcripts of many genes that were identified as being QS regulated.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, Box 672, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642. Phone: (585) 275-4302. Fax: (585) 473-9573. E-mail: bigl{at}mail.rochester.edu.

{dagger} For a commentary on this article, see page 2061 in this issue.

Journal of Bacteriology, April 2003, p. 2080-2095, Vol. 185, No. 7
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.7.2080-2095.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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