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Journal of Bacteriology, April 2003, p. 2227-2235, Vol. 185, No. 7
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.7.2227-2235.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Negative Control of Quorum Sensing by RpoN ({sigma}54) in Pseudomonas aeruginosa PAO1

Karin Heurlier, Valerie Dénervaud, Gabriella Pessi,{dagger} Cornelia Reimmann, and Dieter Haas*

Laboratoire de Biologie Microbienne, Université de Lausanne, CH-1015 Lausanne, Switzerland

Received 24 July 2002/ Accepted 13 January 2003

In Pseudomonas aeruginosa PAO1, the expression of several virulence factors such as elastase, rhamnolipids, and hydrogen cyanide depends on quorum-sensing regulation, which involves the lasRI and rhlRI systems controlled by N-(3-oxododecanoyl)-L-homoserine lactone and N-butyryl-L-homoserine lactone, respectively, as signal molecules. In rpoN mutants lacking the transcription factor {sigma}54, the expression of the lasR and lasI genes was elevated at low cell densities, whereas expression of the rhlR and rhlI genes was markedly enhanced throughout growth by comparison with the wild type and the complemented mutant strains. As a consequence, the rpoN mutants had elevated levels of both signal molecules and overexpressed the biosynthetic genes for elastase, rhamnolipids, and hydrogen cyanide. The quorum-sensing regulatory protein QscR was not involved in the negative control exerted by RpoN. By contrast, in an rpoN mutant, the expression of the gacA global regulatory gene was significantly increased during the entire growth cycle, whereas another global regulatory gene, vfr, was downregulated at high cell densities. In conclusion, it appears that GacA levels play an important role, probably indirectly, in the RpoN-dependent modulation of the quorum-sensing machinery of P. aeruginosa.

* Corresponding author. Mailing address: Institut de Microbiologie Fondamentale, Université de Lausanne, CH-1015 Lausanne, Switzerland. Phone: 41 21 692 56 31. Fax: 41 21 692 56 35. E-mail: Dieter.Haas{at}imf.unil.ch.

{dagger} Present address: Department of Genetics and Developmental Biology, Center for Microbial Pathogenesis, University of Connecticut Health Center, Farmington, CT 06030-3710.

Journal of Bacteriology, April 2003, p. 2227-2235, Vol. 185, No. 7
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.7.2227-2235.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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