This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Schembri, M. A. Articles by Klemm, P. Search for Related Content PubMed PubMed Citation Articles by Schembri, M. A. Articles by Klemm, P.

Differential Expression of the Escherichia coli Autoaggregation Factor Antigen 43

Microbial Adhesion Group, BioCentrum-DTU, Technical University of Denmark, DK-2800 Lyngby, Denmark

Received 2 October 2002/ Accepted 26 December 2002

Antigen 43 (Ag43) is a self-recognizing surface adhesin found in most Escherichia coli strains. Due to its excellent cell-to-cell aggregation characteristics, Ag43 expression confers clumping and fluffing of cells and promotes biofilm formation. Ag43 expression is repressed by the cellular redox sensor OxyR. Here we used mutant versions of OxyR that are locked in either the reduced or the oxidized form as well as the addition of a simple redox-changing chemical to show that the redox state of OxyR influences Ag43 expression. Furthermore, the redox state of OxyR influences the biofilm-forming potential of E. coli. Finally, we demonstrated that Ag43-mediated cell aggregation confers significant protection against hydrogen peroxide killing.

This article has been cited by other articles:

• Geier, H., Mostowy, S., Cangelosi, G. A., Behr, M. A., Ford, T. E. (2008). Autoinducer-2 Triggers the Oxidative Stress Response in Mycobacterium avium, Leading to Biofilm Formation. Appl. Environ. Microbiol. 74: 1798-1804 [Abstract] [Full Text]  
• Ulett, G. C., Valle, J., Beloin, C., Sherlock, O., Ghigo, J.-M., Schembri, M. A. (2007). Functional Analysis of Antigen 43 in Uropathogenic Escherichia coli Reveals a Role in Long-Term Persistence in the Urinary Tract. Infect. Immun. 75: 3233-3244 [Abstract] [Full Text]  
• Restieri, C., Garriss, G., Locas, M.-C., Dozois, C. M. (2007). Autotransporter-Encoding Sequences Are Phylogenetically Distributed among Escherichia coli Clinical Isolates and Reference Strains. Appl. Environ. Microbiol. 73: 1553-1562 [Abstract] [Full Text]  
• Fexby, S., Bjarnsholt, T., Jensen, P. O., Roos, V., Hoiby, N., Givskov, M., Klemm, P. (2007). Biological Trojan Horse: Antigen 43 Provides Specific Bacterial Uptake and Survival in Human Neutrophils. Infect. Immun. 75: 30-34 [Abstract] [Full Text]  
• Young, K. D. (2006). The Selective Value of Bacterial Shape. Microbiol. Mol. Biol. Rev. 70: 660-703 [Abstract] [Full Text]  
• Casadesus, J., Low, D. (2006). Epigenetic Gene Regulation in the Bacterial World. Microbiol. Mol. Biol. Rev. 70: 830-856 [Abstract] [Full Text]  
• Ulett, G. C., Webb, R. I., Schembri, M. A. (2006). Antigen-43-mediated autoaggregation impairs motility in Escherichia coli. Microbiology 152: 2101-2110 [Abstract] [Full Text]  
• Moreira, C. G., Palmer, K., Whiteley, M., Sircili, M. P., Trabulsi, L. R., Castro, A. F. P., Sperandio, V. (2006). Bundle-Forming Pili and EspA Are Involved in Biofilm Formation by Enteropathogenic Escherichia coli. J. Bacteriol. 188: 3952-3961 [Abstract] [Full Text]  
• De Windt, W., Gao, H., Kromer, W., Van Damme, P., Dick, J., Mast, J., Boon, N., Zhou, J., Verstraete, W. (2006). AggA is required for aggregation and increased biofilm formation of a hyper-aggregating mutant of Shewanella oneidensis MR-1.. Microbiology 152: 721-729 [Abstract] [Full Text]  
• Beloin, C., Michaelis, K., Lindner, K., Landini, P., Hacker, J., Ghigo, J.-M., Dobrindt, U. (2006). The Transcriptional Antiterminator RfaH Represses Biofilm Formation in Escherichia coli. J. Bacteriol. 188: 1316-1331 [Abstract] [Full Text]  
• Nachin, L., Nannmark, U., Nystrom, T. (2005). Differential Roles of the Universal Stress Proteins of Escherichia coli in Oxidative Stress Resistance, Adhesion, and Motility. J. Bacteriol. 187: 6265-6272 [Abstract] [Full Text]  
• Sherlock, O., Vejborg, R. M., Klemm, P. (2005). The TibA Adhesin/Invasin from Enterotoxigenic Escherichia coli Is Self Recognizing and Induces Bacterial Aggregation and Biofilm Formation. Infect. Immun. 73: 1954-1963 [Abstract] [Full Text]  
• van der Woude, M. W., Baumler, A. J. (2004). Phase and Antigenic Variation in Bacteria. Clin. Microbiol. Rev. 17: 581-611 [Abstract] [Full Text]  
• Schembri, M. A., Dalsgaard, D., Klemm, P. (2004). Capsule Shields the Function of Short Bacterial Adhesins. J. Bacteriol. 186: 1249-1257 [Abstract] [Full Text]