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Journal of Bacteriology, April 2003, p. 2346-2353, Vol. 185, No. 7
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.7.2346-2353.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Localization of rRNA Synthesis in Bacillus subtilis: Characterization of Loci Involved in Transcription Focus Formation

Karen M. Davies and Peter J. Lewis*

School of Environmental and Life Sciences, Biological Sciences, University of Newcastle, Callaghan, New South Wales 2308, Australia

Received 24 October 2002/ Accepted 14 January 2003

In Bacillus subtilis, RNA polymerase becomes concentrated into regions of the nucleoid called transcription foci. With green fluorescent protein-tagged RNA polymerase, these structures are only observed at higher growth rates and have been shown to represent the sites of rRNA synthesis. There are 10 rRNA (rrn) operons distributed around nearly half of the chromosome. In this study we analyzed the rrn composition of transcription foci with fluorescently tagged loci and showed that they comprise the origin-proximal operon rrnO but not the more dispersed rrnE or rrnD. This suggests that transcription foci comprise only the seven origin-proximal operons rrnO, rrnA, rrnJ, rrnW, rrnI, rrnH, and rrnG. These results have important implications for our understanding of microbial chromosome structure.

* Corresponding author. Mailing address: School of Environmental and Life Sciences, Biological Sciences, University of Newcastle, Callaghan, New South Wales 2308, Australia. Phone: 612 4921 5701. Fax: 612 4921 6923. E-mail: Peter.Lewis{at}newcastle.edu.au.

Journal of Bacteriology, April 2003, p. 2346-2353, Vol. 185, No. 7
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.7.2346-2353.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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  • Doherty, G. P., Meredith, D. H., Lewis, P. J. (2006). Subcellular Partitioning of Transcription Factors in Bacillus subtilis. J. Bacteriol. 188: 4101-4110 [Abstract] [Full Text]  
  • Rocha, E. P. C. (2004). The replication-related organization of bacterial genomes. Microbiology 150: 1609-1627 [Abstract] [Full Text]  


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