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Journal of Bacteriology, April 2003, p. 2369-2373, Vol. 185, No. 7
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.7.2369-2373.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Structural and Functional Analysis of a Putative Gene Cluster for Palatinose Transport on the Linear Chromosome of Agrobacterium tumefaciens MAFF301001

Devika M. De Costa,{dagger} Katsunori Suzuki,* and Kazuo Yoshida*

Department of Biological Science, Graduate School of Science, Hiroshima University, Higashi-Hiroshima 739-8526, Japan

Received 16 September 2002/ Accepted 6 January 2003

We identified a putative pal gene cluster (palR, palE, palF, palG, palK, palA, and palB) in the plant-tumorigenic bacterium Agrobacterium tumefaciens MAFF301001; by sequencing analyses, this cluster was found to be involved in palatinose transport, and its functional importance was revealed by mutational analyses. The pal gene products were highly homologous to those of putative trehalose/maltose ABC-type transport systems but were not essential to bacterial growth on trehalose. Insertion mutations in the palK and palE genes showed the necessity of these genes for bacterial growth and chemotaxis with palatinose as the carbon source, but no inhibition of tumorigenesis was observed. Growth on trehalose and maltose was not influenced by the mutations.

* Corresponding author. Mailing address: Department of Biological Science, Graduate School of Science, Hiroshima University, Kagamiyama 1-3-1, Higashi-Hiroshima 739-8526, Japan. Phone: 81-824-24-7456. Fax: 81-824-24-0734. E-mail for Katsunori Suzuki: ksuzuki{at}hiroshima-u.ac.jp.

* Corresponding author. Mailing address: Department of Biological Science, Graduate School of Science, Hiroshima University, Kagamiyama 1-3-1, Higashi-Hiroshima 739-8526, Japan. Phone: 81-824-24-7456. Fax: 81-824-24-0734. E-mail for Kazuo Yoshida: kzyoshida{at}kamon.ne.jp.

{dagger} Present address: Department of Agricultural Biology, Faculty of Agriculture, University of Peradeniya, Peradeniya, Sri Lanka.

Journal of Bacteriology, April 2003, p. 2369-2373, Vol. 185, No. 7
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.7.2369-2373.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.





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